Journal ArticleDOI
Probing conformational changes in proteins by mass spectrometry
TLDR
The authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.Abstract:
Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.read more
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Journal ArticleDOI
Variable-Temperature ESI-IMS-MS Analysis of Myohemerythrin Reveals Ligand Losses, Unfolding, and a Non-Native Disulfide Bond.
Daniel W. Woodall,Tarick J. El-Baba,Daniel R. Fuller,Wen Liu,Christopher J. Brown,Arthur Laganowsky,David H. Russell,David E. Clemmer +7 more
TL;DR: The high-fidelity of IMS-MS techniques provides a means of examining the stabilities of individual components of complex mixtures that are inaccessible by traditional calorimetric and spectroscopic methods.
Journal ArticleDOI
Influence of acid-induced conformational variability on protein separation in reversed phase high performance liquid chromatography
TL;DR: The results suggest a pore exclusion induced separation related to protein conformation, which is influenced by the pH and type of acid used, and is likely to involve ion-pair formation.
Journal ArticleDOI
Advanced mass spectrometry-based methods for the analysis of conformational integrity of biopharmaceutical products.
TL;DR: The two particularly promising methods that are likely to have the most significant and lasting impact in many areas of biopharmaceutical analysis, direct ESI MS and hydrogen/deuterium exchange, are focus of this article.
Journal ArticleDOI
Gas-Phase Analysis of the Complex of Fibroblast GrowthFactor 1 with Heparan Sulfate : A Traveling Wave Ion Mobility Spectrometry (TWIMS) and Molecular Modeling Study
Yuejie Zhao,Arunima Singh,Yongmei Xu,Chengli Zong,Fuming Zhang,Geert-Jan Boons,Jian Liu,Robert J. Linhardt,Robert J. Woods,I. Jonathan Amster +9 more
TL;DR: This work examined the effect of size and sulfation pattern of HS upon FGF1 oligomerization, binding stoichiometry and conformational stability, through a combination of ion mobility (IM) and theoretical modeling approaches, and demonstrated that certain tetrasaccharide-length fragments are also capable of inducing dimerization of FGF 1.
Journal ArticleDOI
Charge state distribution shifting of protein ions observed in matrix-assisted laser desorption ionization mass spectrometry.
J. Zhou,Terry D. Lee +1 more
TL;DR: A significant shift to lower mass-to-charge values can be obtained for many protein samples by using a new sample preparation method for matrix-assisted laser desorption ionization.
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