Journal ArticleDOI
Probing conformational changes in proteins by mass spectrometry
TLDR
The authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.Abstract:
Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.read more
Citations
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Journal ArticleDOI
Temperature-Controlled Electrospray Ionization: Recent Progress and Applications.
TL;DR: The field of temperature-controlled mass spectrometry has developed over the last thirty years as discussed by the authors, and several nESI and ESI sources capable of controlling spray solution temperature have been developed.
Journal ArticleDOI
The investigation of β-cyclodextrin noncovalent complex with protein or dipeptide by electrospray ionization mass spectrometry
TL;DR: The data obtained from the investigated systems show that ESI‐MS is not only a reliable, sensitive, and fast technique for the analysis of the β‐ CD/protein and β‐CD/dipeptide complexes, but also an interesting tool to observe the transformation from one complex to the other in aqueous solution.
Book ChapterDOI
Electrospray Ionization: Theory and Application
TL;DR: For example, the fast atom bombardment (FAB) method as mentioned in this paper has been used to sample from solution for analytical mass spectrometric analyses of biological molecules, such as peptides, proteins, oligosaccharides and oligonucleotides.
Book ChapterDOI
Chapter 5 Biochemical Reaction Kinetics Studied by Time-Resolved Electrospray Ionization Mass Spectrometry
TL;DR: In this paper, the shape of the age distribution function during data analysis is considered for time-resolved electrospray ionization-mass spectrometry (ESI-MS) data analysis.
Book ChapterDOI
Mass Spectrometry: Structure Determination of Proteins and Peptides
TL;DR: Various strategies involved in large-scale profiling of proteins, including peptide fingerprinting, peptide sequence tag, multidimensional protein identification technology, accurate mass tag, and top-down proteomics are discussed.
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