Journal ArticleDOI
Probing conformational changes in proteins by mass spectrometry
TLDR
The authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.Abstract:
Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.read more
Citations
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Book ChapterDOI
Electrospray Mass Spectrometry
M. Mann,S. Shen,J. B. Fenn +2 more
TL;DR: The electrospray mass spectrometry (ESMS) is a relatively new technique for the analysis of complex, polar, and labile molecules as discussed by the authors, which has attracted widespread interest only since 1988, when it was shown capable of producing and weighing intact ions of very large biomolecules.
Journal ArticleDOI
Characterization of Microdialysis Acidification for Capillary Isoelectric Focusing Microelectrospray Ionization Mass Spectrometry
TL;DR: The microdialysis junction is advantageous over the coaxial liquid sheath interface as evidenced by the simplicity in operation procedures, the enhancement in detection sensitivity, and the linear correlation between protein migration time and isoelectric point in CIEF-ESI-MS.
Journal ArticleDOI
Do proteins denature during droplet evolution in electrospray ionization
Urooj A. Mirza,Brian T. Chait +1 more
TL;DR: In this article, it was shown that it is relatively difficult to induce a change in charge in selected proteins by the application of heat to electrosprayed droplets during their transfer from atmospheric pressure to the vacuum of the mass spectrometer.
Journal ArticleDOI
Conformational dynamics of partially denatured myoglobin studied by time-resolved electrospray mass spectrometry with online hydrogen-deuterium exchange.
TL;DR: This study demonstrates the use of electrospray mass spectrometry in conjunction with rapid online mixing ("time-resolved" ESI-MS) for monitoring protein conformational dynamics under equilibrium conditions.
Journal ArticleDOI
Rectilinear ion trap mass spectrometer with atmospheric pressure interface and electrospray ionization source
Qingyu Song,Sameer Kothari,Michael A Senko,Jae C. Schwartz,Jonathan W. Amy,George C. Stafford,R. Graham Cooks,Zheng Ouyang +7 more
TL;DR: The way in which the ion trapping capacity depends on the dc trapping potential was investigated by measuring the mass shift due to space charge effects, and it was shown that low trapping potentials minimize space charges effects by increasing the useful volume of the device.
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