Journal ArticleDOI
Probing conformational changes in proteins by mass spectrometry
TLDR
The authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.Abstract:
Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.read more
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Journal ArticleDOI
Charge‐state distributuion and electric‐discharge suppression in negative‐ion electrospray mass spectrometry using/chlorinated solvents
Richard B. Cole,A. Kamel Harrata +1 more
TL;DR: In this article, the authors examined solvent effects in electrospray mass spectrometry performed in the negaative-ion mode, for a series of low-nucleophilicity chlorinated solvents.
Journal ArticleDOI
MALDI mass spectrometry of dye-peptide and dye-protein complexes.
Bekir Salih,Renato Zenobi +1 more
TL;DR: It is proved that the interaction between dye-protein and dye-peptide complexes is prodominantly electrostatic and that it involves protonated sites of the peptides and proteins, including the NH2 terminus, and deprotonated SO3 groups of the dyes.
Journal ArticleDOI
Gas-phase processes and measurements of macromolecular properties in solution: On the possibility of false positive and false negative signals of protein unfolding
TL;DR: In this paper, the effect of gas phase processes on mass measurement (e.g., integrity of macromolecular complexes in the absence of solvent, non-specific interactions, etc.), the focus of attention is the ionic charge.
Journal ArticleDOI
Gas Phase Stability of Protein Ions in a Cyclic Ion Mobility Spectrometry Traveling Wave Device.
Charles Eldrid,Jakub Ujma,Symeon Kalfas,Nick Tomczyk,Kevin Giles,Michael Morris,Konstantinos Thalassinos,Konstantinos Thalassinos +7 more
TL;DR: Investigation of protein ion stability using a multipass traveling wave (TW) cyclic IM (cIM) device and the geometry of the instrument provides unique capabilities with the potential to expand the field of protein analysis via IM-MS.
Journal ArticleDOI
Solid-state glycation of β-lactoglobulin monitored by electrospray ionisation mass spectrometry and gel electrophoresis techniques
TL;DR: The charge state distribution data obtained by ESI-MS indicated that adduct formation with lactose, but more significantly galactose, tends to improve the stability properties of the native protein towards denaturation.
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