Journal ArticleDOI
Probing conformational changes in proteins by mass spectrometry
TLDR
The authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.Abstract:
Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.read more
Citations
More filters
Mechanistic insights into the activation of the IKK kinase complex by the Kaposi ’ s sarcoma herpes virus oncoprotein
Claire Bagnéris,Swathi Senthil Kumar,Mehdi Baratchian,Hannah M. Britt,Tufa E. Assafa,Konstantinos Thalassinos,Mary Collins,Tracey E. Barrett +7 more
TL;DR: It is demonstrated here that vFLIP alone is sufficient to activate the IKK kinase complex, and weakly stabilized, high molecular weight vFLip – IKK γ assemblies that are key to the activation process are identified.
Dissertation
The effect of charge and temperature on gas phase protein conformational landscapes : an ion mobility mass spectrometry investigation
TL;DR: In this paper, the authors present a collection of acknowledgements and acknowledgements of the authors of the paper, "Acknowledgements and Acknowledgements: A Declaration and acknowledgements of acknowledgment".
Journal ArticleDOI
Analysis of Megadalton-Sized DNA by Charge Detection Mass Spectrometry: Entropic Trapping and Shearing in Nanoelectrospray.
TL;DR: In this article , the authors used charge detection mass spectrometers to analyze megadalton-sized DNA plasmids and found that the mass distribution shifts to slightly lower mass with increasing charge.
Journal ArticleDOI
Fuzzy Drug Targets: Disordered Proteins in the Drug-Discovery Realm
Suman Saurabh,Karthik Nadendla,Shubh Sanket Purohit,Ponnurengam Malliappan Sivakumar,Sibel Cetinel +4 more
TL;DR: In this article , the authors discuss various experimental and computational techniques that contribute toward understanding IDP "structure" and describe representative pursuances toward IDP-targeting drug development, and also discuss ideas on developing rational drug design protocols targeting IDPs.
Molecular and Biochemical Analysis of the Histidine Kinase CusS and its Role in Metal Resistance in Escherichia coli
TL;DR: Copper sensing by the periplasmic domain of CusS is proposed to initiate signal transduction in the Cus system, an essential cofactor required in many enzymatic processes that require redox changes.
Related Papers (5)
Electrospray ionization for mass spectrometry of large biomolecules
Observation of the heme-globin complex in native myoglobin by electrospray-ionization mass spectrometry
Viswanatham Katta,Brian T. Chait +1 more