Journal ArticleDOI
Probing conformational changes in proteins by mass spectrometry
TLDR
The authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.Abstract:
Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.read more
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Journal ArticleDOI
Investigating the structural transitions of proteins during dissolution by mass spectrometry.
TL;DR: According to the results, cytochrome c underwent significant unfolding during dissolution in the most commonly used NH4Ac buffer, and similar unfolding phenomenon was observed on holo-myoglobin, however, the observed dissolution feature of insulin was different.
Book ChapterDOI
Electrospray Ionization FTICR Spectrometry of Metalloproteins
TL;DR: The capability of mass spectrometry to make measurements for multimeric metalloproteins is investigated, finding it to be able to determine the subunit amino acid composition, the metal stoichiometry, and the sub unit stoichiology of these recombinant metalliproteins.
Journal ArticleDOI
Mechanistic insights into the activation of the IKK kinase complex by the Kaposi’s sarcoma herpes virus oncoprotein vFLIP
Claire Bagnéris,S. Senthil Kumar,Mehdi Baratchian,Hannah M. Britt,Tufa E. Assafa,Konstantinos Thalassinos,Mary Collins,Tracey E. Barrett +7 more
TL;DR: In this paper , a combination of cell-based assays, biophysical techniques, and structural biology was used to demonstrate that vFLIP alone is sufficient to activate the IKK kinase complex.
Caracterización de un proteoma mínimo: Mycoplasma genitalium
TL;DR: Por consiguiente, no solo se presenta el analisis proteomico mas extenso de Mycoplasma genitalium, sino tambien el analizis proteico mas completo de la membrana de mycoplas genitalium ademas de la respuesta serologica in humanos.
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