Journal ArticleDOI
Probing conformational changes in proteins by mass spectrometry
TLDR
The authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.Abstract:
Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.read more
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Journal ArticleDOI
Electrospray vs. matrix-assisted laser desorption/ionization. 1. Some examples in the protein field
TL;DR: In this paper, the relative molecular mass (RMM) of proteins in the range 1150-29000 was examined by means of both positive electrospray and matrix-assisted laser desorption/ionization mass spectrometry.
Journal ArticleDOI
Ion mobility spectrometry focusing on speciation analysis of metals/metalloids bound to carbonic anhydrase
TL;DR: Using TWIMS-MS, two conformational states of bovine carbonic anhydrase were observed with charge states of +12 and +11; these configurations being evaluated in terms of the folded state of the apo form and this protein being linked to barium, lead, copper, and zinc in their divalent forms.
Journal ArticleDOI
Engineering an autonomous VH domain to modulate intracellular pathways and to interrogate the eIF4F complex
Yuri Frosi,Yen-Chu Lin,Jiang Shimin,Siti Radhiah Ramlan,Kelly Hew,Alf Henrik Engman,Anil Pillai,Kit Yeung,Yue Xiang Cheng,Tobias Cornvik,Pär Nordlund,Megan Goh,Dilraj Lama,Zachary P. Gates,Chandra Varma,Dawn Thean,David P. Lane,Ignacio Asial,C.J. Brown +18 more
TL;DR: In this paper , an autonomous and disufhide-free VH domain is used for intracellular studies and used it to construct a high-diversity phage display library.
Journal ArticleDOI
Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers
TL;DR: Glycerol carbonate was synthesized, which ionizing and dissociating abilities were very close to those of water and suggested that GC is a potential biosolvent, which has great significance to biocatalysis in nonaqueous solvents.
Journal ArticleDOI
Characterisation of cytochrome c unfolding by nano-electrospray ionization time-of-fight and Fourier transform ion cyclotron resonance mass spectrometry.
TL;DR: The effect of trifluoroethanol (TFE) on cytochrome c equilibrium unfolding at different pH by nano-ESI-MS is described and general good agreement in conformational effects revealed by the different instruments under several buffer conditions is revealed.
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