Production, purification, and characterization of recombinant maspin proteins.
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TLDR
Evidence that recombinant maspin protein itself inhibits invasion, and supports the role of maspIn as a tumor suppressor, is provided.About:
This article is published in Journal of Biological Chemistry.The article was published on 1994-12-09 and is currently open access. It has received 167 citations till now. The article focuses on the topics: Maspin & Fusion protein.read more
Citations
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Maspin is an angiogenesis inhibitor.
TL;DR: The data suggest that the tumor suppressor activity of maspin may depend in large part on its ability to inhibit angiogenesis and raise the possibility that maspIn and similar serpins may be excellent leads for the development of drugs that modulateAngiogenesis.
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Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells
TL;DR: Re recombinant maspin protein blocks the motility of mammary carcinoma cells in culture over 12 h, as demonstrated by time-lapse video microscopy.
Journal Article
The human myoepithelial cell is a natural tumor suppressor.
TL;DR: The cumulative data suggest that myoepithelial cells are natural paracrine suppressors of invasion and metastasis and may specifically inhibit the progression of precancerous disease states to invasive cancer in vivo.
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A novel protease homolog differentially expressed in breast and ovarian cancer.
Anthony Anisowicz,Georgia Sotiropoulou,Georgia Sotiropoulou,Göran Stenman,Samuel C. Mok,Ruth Sager +5 more
TL;DR: Protease M expression (mRNA) may be a useful marker in the detection of primary mammary carcinomas, as well as primary ovarian cancers, and other medical applications are also likely, based on sequence relatedness to trypsin and PSA.
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Expression genetics in cancer: shifting the focus from DNA to RNA.
TL;DR: The authors' success in readily isolating more than 100 candidate tumor suppressor genes from human tissue is reported, estimated to represent roughly 20% of the total genes recoverable by this approach.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Book
Antibodies: A Laboratory Manual
Ed Harlow,David P. Lane +1 more
TL;DR: A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.
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Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
TL;DR: Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.
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Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells
Zhiqiang Zou,Anthony Anisowicz,Mary J.C. Hendrix,Ann D. Thor,Mark Joseph Neveu,Shijie Sheng,Kristina Rafidi,Elisabeth A. Seftor,Ruth Sager +8 more
TL;DR: Results support the hypothesis that maspin functions as a tumor suppressor and reduce the cells' ability to induce tumors and metastasize in nude mice and to invade through a basement membrane matrix in vitro.