Journal ArticleDOI
Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells
Zhiqiang Zou,Anthony Anisowicz,Mary J.C. Hendrix,Ann D. Thor,Mark Joseph Neveu,Shijie Sheng,Kristina Rafidi,Elisabeth A. Seftor,Ruth Sager +8 more
TLDR
Results support the hypothesis that maspin functions as a tumor suppressor and reduce the cells' ability to induce tumors and metastasize in nude mice and to invade through a basement membrane matrix in vitro.Abstract:
A gene encoding a protein related to the serpin family of protease inhibitors was identified as a candidate tumor suppressor gene that may play a role in human breast cancer. The gene product, called maspin, is expressed in normal mammary epithelial cells but not in most mammary carcinoma cell lines. Transfection of MDA-MB-435 mammary carcinoma cells with the maspin gene did not alter the cells' growth properties in vitro, but reduced the cells' ability to induce tumors and metastasize in nude mice and to invade through a basement membrane matrix in vitro. Analysis of human breast cancer specimens revealed that loss of maspin expression occurred most frequently in advanced cancers. These results support the hypothesis that maspin functions as a tumor suppressor.read more
Citations
More filters
Journal ArticleDOI
Putting tumours in context
Mina J. Bissell,Derek C. Radisky +1 more
TL;DR: In this article, the interactions between cancer cells and their micro-and macro-environment create a context that promotes tumour growth and protects it from immune attack, and the functional association of cancer cells with their surrounding tissues forms a new 'organ' that changes as malignancy progresses.
Journal ArticleDOI
Predicting the clinical status of human breast cancer by using gene expression profiles.
Mike West,Carrie Blanchette,Holly K. Dressman,Erich Huang,Seiichi Ishida,Rainer Spang,Harry Zuzan,John A. Olson,Jeffrey R. Marks,Joseph R. Nevins +9 more
TL;DR: Bayesian regression models that provide predictive capability based on gene expression data derived from DNA microarray analysis of a series of primary breast cancer samples are developed and the utility and validity of such models in predicting the status of tumors in crossvalidation determinations are assessed.
Journal ArticleDOI
The serpins are an expanding superfamily of structurally similar but functionally diverse proteins - Evolution, mechanism of inhibition, novel functions, and a revised nomenclature
Gary A. Silverman,Phillip I. Bird,Robin W. Carrell,Frank C. Church,Paul Bernard Coughlin,Peter G.W. Gettins,James A. Irving,David A. Lomas,Cliff J. Luke,Richard W. Moyer,Philip A. Pemberton,Eileen Remold-O'Donnell,Guy S. Salvesen,James Travis,James C. Whisstock +14 more
TL;DR: This work aims to provide a systematic literature review and meta-analyses of the determinants of blood clotting disorders and their Kessler’s disease progression as well as some of the mechanisms behind these reactions.
Journal ArticleDOI
The serpin superfamily of proteinase inhibitors: structure, function, and regulation.
Journal ArticleDOI
Terminal Differentiation of Human Breast Cancer through PPARγ
Elisabetta Mueller,Pasha Sarraf,Peter Tontonoz,Peter Tontonoz,Ronald M. Evans,Ronald M. Evans,Katherine J. Martin,Ming Zhang,Christopher D.M. Fletcher,Christopher D.M. Fletcher,Samuel Singer,Samuel Singer,Bruce M. Spiegelman +12 more
TL;DR: The data suggest that the PPAR gamma transcriptional pathway can induce terminal differentiation of malignant breast epithelial cells and thus may provide a novel, nontoxic therapy for human breast cancer.
References
More filters
Book
Antibodies: A Laboratory Manual
Ed Harlow,David P. Lane +1 more
TL;DR: A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.
Journal ArticleDOI
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
TL;DR: The sequence CCAGCCAUG (G) thus emerges as a consensus sequence for eukaryotic initiation sites, and the extent to which the ribosome binding site in a given mRNA matches the -1 to -5 consensus sequence varies.
Journal ArticleDOI
Implications of the three-dimensional structure of alpha 1-antitrypsin for structure and function of serpins.
Robert Huber,Carrell Rw +1 more
TL;DR: Article synthese sur l'α1-antitrypsine: caracteristiques structurales and fonctionnelles (capacite de liaison a des modulateurs) andrology en tant que modele pour toutes les serpines.
Journal ArticleDOI
Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains.
TL;DR: The amplification procedure, together with the availability of avidin probes labeled with fluorochromes, colloidal gold, or enzyme systems other than HRP, provides a means of greatly increasing the versatility of a variety of histochemical reactions, including those for detecting in situ hybridization probes, in addition to increasing the sensitivity of the reactions.
Journal Article
Tumorigenicity and metastasis of human breast carcinoma cell lines in nude mice.
TL;DR: The results suggest that the estrogen receptor-negative MDA-MB-435 cell line injected into nude mice is a favorable site for the growth of human breast carcinomas and could be a valuable tool for analysis of the cellular and molecular basis of the metastasis of advanced breast cancer.