Journal ArticleDOI
Products of photosynthesis by marine phytoplankton: Chitin in TCA “protein” precipitates☆
Richard A. Smucker,Rodger Dawson +1 more
TLDR
Extension of the standard extraction procedure for phytoplankton photosynthetic carbon pools shows the presence of chitin (poly N- acetyl- d -glucosamine) in the previously considered protein pool, which has been previously characterized solely as the “protei” fraction.About:
This article is published in Journal of Experimental Marine Biology and Ecology.The article was published on 1986-12-31. It has received 44 citations till now. The article focuses on the topics: Thalassiosira pseudonana & Chitin.read more
Citations
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Book ChapterDOI
The Ecology of Chitin Degradation
TL;DR: Gooday et al. as discussed by the authors showed that the (1→4)-β-linked homopolymer of N-acetyl-D-glucosamine can be stabilised as a rigid ribbon by a statistical mixture of CH2OH orientations, equivalent to half oxygens on each residue.
BookDOI
Microbial enzymes in aquatic environments
TL;DR: This volume brings together studies on enzymatic degradation processes from disciplines as diverse as water and sediment research, bacterial and algal aquatic ecophysiology, eutrophication, and nutrient cycling and biogeochemistry, in both freshwater and marine ecosystems.
Journal ArticleDOI
Chitinases from uncultured marine microorganisms.
TL;DR: The results suggest that culture-dependent methods do not greatly underestimate the portion of marine bacterial communities capable of chitin degradation, and suggest a greater appreciation for the genes encoding enzymes involved in the hydrolysis of biopolymers such as Chitin, one of the most abundant polymers in nature.
Journal ArticleDOI
Nitrogen and carbon isotopic ratios of chlorophyll from marine phytoplankton
TL;DR: The relationship between the nitrogen and carbon isotopic ratios of chlorophyll and total biomass was explored in cultured marine phytoplankton to assess the utility of the plant as an isotopic proxy for photoautotrophs as discussed by the authors.
Journal ArticleDOI
Influence of irradiance and spectral quality on the carbon metabolism of phytoplankton I. Photosynthesis, chemical composition and growth
TL;DR: Simulated in situ incubations which adjust irradiance but not spectral quality to in situ levels may not accurately estimate in situ rates of carbon uptake and protein metabolism, and clearly show the importance of considering spectral quality in models of photoadaptation and primary production.
References
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Book ChapterDOI
Culture of Phytoplankton for Feeding Marine Invertebrates
TL;DR: The methods suffice for the most fastidious algae now routinely cultivable, and simplifications indicated for less demanding species are easily made; for example, omission of silicate for plants other than diatoms.
Journal ArticleDOI
A rapid and sensitive assay for chitinase using tritiated chitin.
TL;DR: Radioactive chitin, prepared by acetylation of chitosan with tritiated acetic anhydride, was used as substrate in a rapid and extremely sensitive assay for chitInase, a result that cannot be attributed to an artifact of the method, to inhibition by product, or to instability of the enzyme.
Journal ArticleDOI
The chitinase system of a strain of Streptomyces griseus.
L R Berger,D M Reynolds +1 more
TL;DR: The chitInase system of a strain of Streptomyces griseus was resolved into two chitinase with similar enzymic activities and a chitobiase, which is a heat-labile enzyme which can be irreversibly adsorbed on bauxite.
Journal ArticleDOI
Products of photosynthesis by marine phytoplankton: the effect of environmental factors on the relative rates of protein synthesis
TL;DR: Preliminary experiments have established the suitability of this fractionation method for natural populations of phytoplankton and have shown similar effects of light intensity on the relative rates of protein synthesis.