Journal ArticleDOI
Protective effect of verbascoside in activated C6 glioma cells: possible molecular mechanisms
Emanuela Esposito,Roberto Dal Toso,Giovanna Pressi,Placido Bramanti,Rosaria Meli,Salvatore Cuzzocrea +5 more
TLDR
The mechanisms underlying in vitro the neuroprotective properties of VB involve modulation of transcription factors and consequent altered gene expression, resulting in downregulation of inflammation, providing support that VB may provide a promising approach for the treatment of oxidative-stress-related neurodegenerative diseases.Abstract:
The glycosylated phenylpropanoid verbascoside (VB), isolated from cultured cells of the medicinal plant Syringa vulgaris (Oleaceae), has previously been characterized as an effective scavenger of biologically active free radicals and an inhibitor of lipid peroxidation. The aim of the present study was to evaluate in a rat glioma cell line (C6) the effect of VB biotechnologically produced by S. vulgaris plant cell cultures in the regulation of the inflammatory response. We used a model of central nervous system inflammation induced by bacterial endotoxin/cytokine (lipopolysaccharide (LPS)/interferon (IFN)-gamma, 1 microg/ml and 100 U/ml, respectively). Our results show that the treatment with LPS/IFN-gamma for 24 h elicited the induction of inducible nitric oxide synthase (iNOS) activity as determined by NO(x) accumulation in the culture medium. Preincubation with VB (10-100 microg/ml) abrogated the mixed cytokine-mediated induction of iNOS. The effect was concentration-dependent. Our studies also showed an inhibitory effect of VB on neuronal nitric oxide synthase expression. Moreover, Western blot analysis showed that this glycoside prevents specifically the activation of the proinflammatory enzyme cyclooxygenase (COX)-2 in glioma cells without simultaneous inhibition of COX-1 enzyme. Moreover, we found that VB reduced the expression of proinflammatory enzymes in LPS/IFN-gamma through the inhibition of the activation of nuclear factor kappa B and mitogen-activated protein kinase signaling pathway. The mechanisms underlying in vitro the neuroprotective properties of VB involve modulation of transcription factors and consequent altered gene expression, resulting in downregulation of inflammation. These findings provide support that VB may provide a promising approach for the treatment of oxidative-stress-related neurodegenerative diseases.read more
Citations
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Journal ArticleDOI
Verbascoside — A review of its occurrence, (bio)synthesis and pharmacological significance
TL;DR: Verbascoside is a phenylethanoid glycoside that was first isolated from mullein but is also found in several other plant species as mentioned in this paper, including genetically transformed roots.
Journal ArticleDOI
Polyphenol compounds and PKC signaling
TL;DR: This article reviews PKC-polyphenol interactions and its relevance to various disease states and highlights some polyphenols exert their antioxidant properties by regulating the transcription of the antioxidant enzyme genes through PKC signaling.
Book ChapterDOI
Pharmacology of Olive Biophenols
Hassan K. Obied,Paul D. Prenzler,Syed Haris Omar,Rania Ismael,Maurizio Servili,Sonia Esposto,Agnese Taticchi,Roberto Selvaggini,Stefania Urbani +8 more
TL;DR: The chemistry, pharmacodynamics, pharmacokinetics, posology, adverse effects, and potential drug interactions of olives and major olive biophenols (OBP) are discussed in this paper.
Journal ArticleDOI
Verbascoside promotes apoptosis by regulating HIPK2–p53 signaling in human colorectal cancer
Lihong Zhou,Yuanyuan Feng,Yongjie Jin,Xuan Liu,Hua Sui,Ni Chai,Xingzhu Chen,Ningning Liu,Qing Ji,Yan Wang,Qi Li +10 more
TL;DR: HIPK2 protein modulates the phosphorylation status of p53, and levels of Bax and Bcl-2 in CRC, and it is found that VB effectively activated the HIPK2–p53 signaling pathway, resulting in increased CRC cell apoptosis.
Journal ArticleDOI
Neuroprotective Phenolics in Medicinal Plants
TL;DR: In this review, naturally-occuring neuroprotective phenolics and their underlying mechanisms of neuroProtective actions are summarized.
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