Journal ArticleDOI
Protein determination for large numbers of samples.
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This article is published in Analytical Chemistry.The article was published on 1959-05-01. It has received 1589 citations till now.read more
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A simplification of the protein assay method of Lowry et al. which is more generally applicable
TL;DR: A simple method based on a linear log-log protein standard curve is presented to permit rapid and totally objective protein analysis using small programmable calculators.
Journal ArticleDOI
Determination of protein: A modification of the lowry method that gives a linear photometric response
TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.
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A simplified method for the quantitative assay of small amounts of protein in biologic material.
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Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall.
TL;DR: The method of Lowry and coworkers combined the use of copper, as suggested by Herriott, with the Folin phenol method, which originated from the work of Wu, to produce a more reliable and sensitive protein analysis.
Journal ArticleDOI
Activation of adenylate cyclase by the diterpene forskolin does not require the guanine nucleotide regulatory protein.
TL;DR: Forskolin, a novel diterpene activator of adenylate cyclase in membranes and intact cells, activates the enzyme in membranes from mutant cyc-S49 murine lymphoma cells and the soluble enzyme from rat testes.
References
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Fluorometric determination of uranium
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