Journal ArticleDOI
Purification and characterization of an alkaline pectin lyase from Aspergillus flavus
TLDR
An alkaline pectin lyase secreted by Aspergillus flavus MTCC 7589 was purified to electrophoretic homogeneity using ammonium sulphate fractionation, anion exchange chromatography on DEAE cellulose and gel filtration chromatography in order to show efficacy in retting of Crotalaria juncea fibers.About:
This article is published in Process Biochemistry.The article was published on 2008-05-01. It has received 67 citations till now. The article focuses on the topics: Pectin lyase & Ammonium.read more
Citations
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Journal ArticleDOI
Culture optimization for enhanced production of microbial pectin methylesterase under submerged conditions.
Journal ArticleDOI
Purification, characterization and retting of Crotolaria juncea fibres by an alkaline pectin lyase from Fusarium oxysporum MTCC 1755
TL;DR: The purified pectin lyase was found suitable for retting of Crotolaria juncea fiber and was found to be highly stimulated by Ca2+ ions while sugars like mannitol and sorbitol, and salts like NaCl and CaCl2 enhanced the thermostability.
Journal ArticleDOI
Pectinolytic lyases: a comprehensive review of sources, category, property, structure, and catalytic mechanism of pectate lyases and pectin lyases
TL;DR: Pectate lyases and pectin lyases have essential roles in various biotechnological applications, such as textile industry, paper making, pectic wastewater pretreatment, juice clarification and oil extraction.
Journal ArticleDOI
Purification of pectinases from Aspergillus niger ATCC 9642 by ethanol precipitation
Márcia M. Santin Trentini,Geciane Toniazzo,Jamile Zeni,Jonaina Pili,Marco Di Luccio,Eunice Valduga +5 more
TL;DR: The precipitation method using ethanol was proven to be effective in the recovery and fractionation of pectinases.
Journal ArticleDOI
Characterization of an Alkaline Thermostable Pectin Lyase from Newly Isolated Aspergillus niger _WHAK1 and Its Application on Fruit Juice Clarification
TL;DR: Alkaline thermostable pectin lyase from Aspergillus niger strain_WHAK1 was produced on wheat bran and citrus pect in submerged culture, and it was effective for clarification of fruit juices.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Book
Microbiology : A Laboratory Manual
TL;DR: In this article, basic laboratory techniques for isolation, cultivation, and cultural characterisation of microorganisms are described, including basic techniques for isolating, culturing, and characterizing microorganisms.