Journal ArticleDOI
Purification and characterization of an alkaline pectin lyase from Aspergillus flavus
TLDR
An alkaline pectin lyase secreted by Aspergillus flavus MTCC 7589 was purified to electrophoretic homogeneity using ammonium sulphate fractionation, anion exchange chromatography on DEAE cellulose and gel filtration chromatography in order to show efficacy in retting of Crotalaria juncea fibers.About:
This article is published in Process Biochemistry.The article was published on 2008-05-01. It has received 67 citations till now. The article focuses on the topics: Pectin lyase & Ammonium.read more
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Journal ArticleDOI
Production and partial purification of pectin lyase by Aspergillus niger grown on orange peels
Sidra Batool,M. Javaid Asad,S. M. Saqlan Naqvi Raja Tahir Mahmood,A. Guffar,Muhammad Gulfraz,Saqib Hussain Hadri +5 more
TL;DR: The peel of citrus fruits contains a large percentage of pectin which can be a good substrate for pectinolytic microorganisms and these microbes secrete large amount of extracellular enzymes to degrade the cell wall of substrates.
Journal ArticleDOI
Characterization of a neutral pectin lyase produced by Oidiodendron echinulatum MTCC 1356 in solid state fermentation.
TL;DR: A neutral pectin lyase produced by a new fungal strain Oidiodendron echinulatum MTCC 1356 under solid state fermentation using wheat bran as agro waste has been studied and showed retting of Crotalaria juncea fibre in the presence of EDTA.
Journal ArticleDOI
Production, purification and characterization of pectin lyase from Bacillus subtilis isolated from moong beans leaves (Vigna radiata)
TL;DR: First time alkalophilic pectin lyase form a bacterium isolated from diseased plants is described, described in the best of the authors' knowledge.
Journal Article
Separation,Purification and Characterization of Three Endo-polygalacturonases from a Newly Isolated Penicillum oxalicum
TL;DR: Three endo-polygalacturonases from a newly isolated Penicillum oxalicum capable of utilizing waste biomass as growth substrate were separated and purified to homogeneity by ultra-filtration, affinity adsorption chromatography, CM-cellulose column Chromatography, and Sephadex G-100 gel filtration chromatography with the overall yield of 64.5% from the crude enzyme.
Journal ArticleDOI
XYLANASES AND PECTINASES OF Aspergillus flavus CECT-2687 ON DIFFERENT CARBON SOURCES AND INITIAL pH VALUES
TL;DR: Results shown that this production of xylanases and pectinases is strongly dependent on the initial pH of the culture (is better at pH values between 6 and 8), as much as the composition and availability of polysaccharides used as carbon source.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Book
Microbiology : A Laboratory Manual
TL;DR: In this article, basic laboratory techniques for isolation, cultivation, and cultural characterisation of microorganisms are described, including basic techniques for isolating, culturing, and characterizing microorganisms.