Purification of polyphosphoinositides by chromatography on immobilized neomycin
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TLDR
The binding of polyphosphoinositides to the antibiotic neomycin is utilized for the purification of these lipids and the lipid is completely eluted by the addition of either ammonia or HC1 to the solvent.About:
This article is published in Journal of Lipid Research.The article was published on 1978-11-01 and is currently open access. It has received 304 citations till now. The article focuses on the topics: Column chromatography & Phosphatidylinositol.read more
Citations
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Activation of the zeta isozyme of protein kinase C by phosphatidylinositol 3,4,5-trisphosphate.
TL;DR: The results suggest that PKC zeta may be a target for PIP3 and thus may be involved in the signaling mechanism(s) for growth factors and oncogenes that increase phosphatidylinositol 3-kinase activity.
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Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase.
TL;DR: Evidence that mast cell secretion is inhibited by internalized neomycin, a compound known to interact with PPI, and the PPI phosphodiesterase of human neutrophil plasma membranes can be activated simply by adding GTP analogues in the presence of concentrations of Ca2+ that pertain in unstimulated cells are provided.
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N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases.
TL;DR: It is concluded that N‐WASP transmits signals from tyrosine kinases to cause a polarized rearrangement of cortical actin filaments dependent on PIP2.
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Cytosolic heparin inhibits muscarinic and alpha-adrenergic Ca2+ release in smooth muscle. Physiological role of inositol 1,4,5-trisphosphate in pharmacomechanical coupling.
TL;DR: It is shown that in smooth muscle permeabilized with beta-escin, one of the saponin esters, alpha 1-adrenergic and muscarinic agonists, as well as caffeine and InsP3, cause contractions mediated by Ca2+ release, which supports the conclusion that InsP 3 is the major physiological messenger of the Ca 2+ release component of pharmacomechanical coupling.
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Inositol 1,4,5-trisphosphate: a possible chemical link in excitation-contraction coupling in muscle.
TL;DR: It is proposed that InsP3 acts as a chemical second messenger between transverse (T)-tubular membrane depolarization and calcium release from the sarcoplasmic reticulum in skeletal muscle.
References
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A simple, specific spray for the detection of phospholipids on thin-layer chromatograms.
John C. Dittmer,Robert L. Lester +1 more
TL;DR: A chemical reagent spray specific for phosphate esters, based on molybdenum blue, gives an instantaneous, specific reaction with phospholipids on silica gel or alumina thin layer chromatography (TLC) plates.
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Acetylcholine increases the breakdown of triphosphoinositide of rabbit iris muscle prelabelled with [32P] phosphate
TL;DR: The presence of triphosphoinositide phosphomonoesterase, the enzyme that might be stimulated in the iris smooth muscle by the neurotransmitter, was demonstrated and, under the methods of homogenization and assay, more than 80% of its activity was localized in the particulate fraction.
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Inhibition by neomycin of polyphosphoinositide turnover in subcellular fractions of guinea-pig cerebral cortex in vitro.
TL;DR: It is suggested that the effects of neomycin on polyphosphoinositide metabolism result from the formation of an ionic complex between the lipids and the antibiotic.
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Ion exchange chromatography of intact brain phosphoinositides on diethylaminoethyl cellulose by gradient salt elution in a mixed solvent system.
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Complete fractionation of brain cephalin; isolation from it of phosphatidyl serine, phosphatidyl ethanolamine, and diphosphoinositide.
TL;DR: Detailed proof that inositol phosphatide is a mixture is found and a method for the separation from it of phosphatidyl ethanolamine, phosphatIDyl serine, and a new inositl-containing phosphatides to which the name of diphosphoinositide has been given is described.