Cytosolic heparin inhibits muscarinic and alpha-adrenergic Ca2+ release in smooth muscle. Physiological role of inositol 1,4,5-trisphosphate in pharmacomechanical coupling.
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TLDR
It is shown that in smooth muscle permeabilized with beta-escin, one of the saponin esters, alpha 1-adrenergic and muscarinic agonists, as well as caffeine and InsP3, cause contractions mediated by Ca2+ release, which supports the conclusion that InsP 3 is the major physiological messenger of the Ca 2+ release component of pharmacomechanical coupling.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-10-25 and is currently open access. It has received 329 citations till now. The article focuses on the topics: Carbachol & Myosin phosphatase activity.read more
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Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension
Masayoshi Uehata,Toshimasa Ishizaki,Hiroyuki Satoh,Takashi Ono,Toshio Kawahara,Tamami Morishita,Hiroki Tamakawa,Keiji Yamagami,Jun Inui,Midori Maekawa,Shuh Narumiya +10 more
TL;DR: Pyridine derivative Y-27632 consistently suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells and dramatically corrects hypertension in several hypertensive rat models, suggesting that compounds that inhibit this process might be useful therapeutically.
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Signal transduction and regulation in smooth muscle
Andrew P. Somlyo,Avril V. Somlyo +1 more
TL;DR: Abnormalities of these regulatory mechanisms and isoform variations may contribute to diseases of smooth muscle, and the G-protein-coupled inhibition of protein phosphatase is also likely to be impor-tant in regulating non-muscle cell functions mediated by cytoplasmic myosin II.
Journal ArticleDOI
Muscarinic receptors--characterization, coupling and function.
TL;DR: The actions of muscarinic receptors on the heart, smooth muscle, glands and on neurons (both presynaptic and postsynaptic) in the autonomic nervous system and the central nervous system are analyzed in terms of subtypes, biochemical mechanisms and effects on ion channels, including K+ channels and Ca2+ channels.
Journal Article
Calcium Movements, Distribution, and Functions in Smooth Muscle
Hideaki Karaki,Hiroshi Ozaki,Masatoshi Hori,Minori Mitsui-Saito,Ken-ichi Amano,Ken-ichi Harada,Shigeki Miyamoto,Hiroshi Nakazawa,Kyung-Jong Won,Koichi Sato +9 more
TL;DR: Contraction of smooth muscle is regulated by the cytosolic Ca2+ level ([Ca2+]i)b, and the sensitivity of the contractile elements in response to changes in the environment surrounding the cell.
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Rho-associated Kinase Directly Induces Smooth Muscle Contraction through Myosin Light Chain Phosphorylation
Yasuko Kureishi,Sei Kobayashi,Mutsuki Amano,Kazushi Kimura,Hideo Kanaide,Takeshi Nakano,Kozo Kaibuchi,Masaaki Ito +7 more
TL;DR: It is demonstrated that Rho-kinase directly modulates smooth muscle contraction through myosin light chain phosphorylation, independently of the Ca2+-calmodulin-dependent myos in light chain kinase pathway.
References
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A new generation of Ca2+ indicators with greatly improved fluorescence properties.
TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.
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Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase.
TL;DR: Evidence that mast cell secretion is inhibited by internalized neomycin, a compound known to interact with PPI, and the PPI phosphodiesterase of human neutrophil plasma membranes can be activated simply by adding GTP analogues in the presence of concentrations of Ca2+ that pertain in unstimulated cells are provided.
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Ryanodine activation and inhibition of the Ca2+ release channel of sarcoplasmic reticulum.
TL;DR: The results suggested two possible modes of action of ryanodine: 1) a change in the gating mechanism of the channel which is not readily detected using the slowly permeating molecule L-glucose or 2) achange in channel structure which prevents its complete closing.
Journal Article
Electromechanical and pharmacomechanical coupling in vascular smooth muscle
Avril V. Somlyo,Andrew P. Somlyo +1 more
TL;DR: The existence of two major electrophysiologic types of vascular smooth muscle, one gradedly responsive and the other producing repetitive action potentials, is suggested.
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Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium.
TL;DR: Results suggest that actions of inositol 1,4,5-trisphosphate are regulated by physiological alterations in intracellular pH and calcium concentrations.