Journal ArticleDOI
Quartz crystal microbalance for the detection of microgram quantities of human serum albumin: relationship between the frequency change and the mass of protein adsorbed
Makoto Muratsugu,Fumihiko. Ohta,Yoshihiro. Miya,Toshiaki Hosokawa,Shigeru Kurosawa,Naoki Kamo,Hisami Ikeda +6 more
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TLDR
The immunosensor did not respond to bovine serum albumin, only to HSA, implying that the specificity for HSA was high, and the relationship between the frequency change and adsorption per unit area of piezoelectrically active quartz crystal was investigated.Abstract:
We have developed a piezoelectric immunosensor for the detection of microalbumin. Human serum albumin (HSA) in the range 0.1-100 micrograms mL-1 could be detected using a flow cell; the immunosensor is sensitive enough to monitor levels of albuminuria. The immunosensor did not respond to bovine serum albumin, only to HSA, implying that the specificity for HSA was high. We investigated the relationship between the frequency change (delta F) and adsorption per unit area of piezoelectrically active quartz crystal (delta M). delta M was estimated with radioisotope-labeled anti-HSA or HSA. When anti-HSA was adsorbed onto the surface of the crystal or HSA was bound to anti-HSA supported by the crystal, values of magnitude of delta F/delta M were larger than the value predicted from theory (Sauerbrey's equation). Furthermore, magnitude of delta F/delta M for HSA was larger than that for anti-HSA.read more
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Variations in Coupled Water, Viscoelastic Properties, and Film Thickness of a Mefp-1 Protein Film during Adsorption and Cross-Linking: A Quartz Crystal Microbalance with Dissipation Monitoring, Ellipsometry, and Surface Plasmon Resonance Study
TL;DR: It is shown how quantitative data about the thickness, shear elastic modulus, and shear viscosity of the protein film can be obtained with the QCM-D technique, even beyond the Sauerbrey regime, if frequency (f) and energy dissipation (D) measurements measured at multiple harmonics are combined with theoretical simulations using a Voight-based viscoelastic model.
Journal ArticleDOI
A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalance/dissipation
Fredrik Höök,Janos Vörös,M. Rodahl,R. Kurrat,Peter Böni,Jeremy J. Ramsden,Marcus Textor,Nicholas D. Spencer,Pentti Tengvall,J. Gold,Bengt Herbert Kasemo +10 more
TL;DR: In this article, the adsorption kinetics of three model proteins (human serum albumin, fibrinogen and hemoglobin) were measured and compared using three different experimental techniques: optical waveguide lightmode spectroscopy (OWLS), ellipsometry (ELM) and quartz crystal microbalance (QCM-D).
Journal ArticleDOI
Commercial quartz crystal microbalances-Theory and applications
TL;DR: A tutorial review article on the quartz crystal microbalance (QCM) is presented in this paper, followed by a summary of recent and important prior research in each of the different areas of analytical interest in the QCM.
Journal ArticleDOI
Piezoelectric Mass‐Sensing Devices as Biosensors—An Alternative to Optical Biosensors?
TL;DR: The future will show whether the quartz-crystal microbalance will assert itself against established label-free sensor devices such as surface plasmon resonance spectroscopy and interferometry.
Journal ArticleDOI
Energy Dissipation Kinetics for Protein and Antibody−Antigen Adsorption under Shear Oscillation on a Quartz Crystal Microbalance
TL;DR: In this article, a new quartz crystal microbalance instrument, allowing simultaneous frequency (f) and dissipation factor (D) measurements, has been used to study protein adsorption kinetics by measuring time-resolved data of both the D-factor, measuring energy dissipation due to the added overlayer, and the f-shift, measuring the effective mass load on the sensor.
References
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Journal ArticleDOI
Verwendung von Schwingquarzen zur Wägung dünner Schichten und zur Mikrowägung
TL;DR: In this paper, eigenfrequenz der Platte infolge Vergroserung der schwingenden Masse is vermessen, so das eine empirische Eichung bei der Schichtwagung mit Schwingquarzen entfallt.
Journal ArticleDOI
Use of quartz vibration for weighing thin films on a microbalance
G. Sauerbrey,F. Sauerbrey +1 more
Journal ArticleDOI
Microalbuminuria Predicts Clinical Proteinuria and Early Mortality in Maturity-Onset Diabetes
TL;DR: It is concluded that microalbuminuria in patients with Type II diabetes is predictive of clinical proteinuria and increased mortality.
Journal ArticleDOI
Frequency of a quartz microbalance in contact with liquid
Journal ArticleDOI
Experimental aspects of use of the quartz crystal microbalance in solution
Stanley Bruckenstein,M. Shay +1 more
TL;DR: TTL-based circuitry for measuring the resonant frequency of an oscillating quartz crystal having one face in contact with an electrolytic solution is described in this article, where the effect of double layer structure and specific adsorption is discussed.
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