Showing papers in "Colloids and Surfaces B: Biointerfaces in 2002"

TL;DR: In this article, the adsorption kinetics of three model proteins (human serum albumin, fibrinogen and hemoglobin) were measured and compared using three different experimental techniques: optical waveguide lightmode spectroscopy (OWLS), ellipsometry (ELM) and quartz crystal microbalance (QCM-D).

TL;DR: In this paper, the surface compositions of four industrial spray-dried dairy powders (skim milk, whole milk powder, cream powder and whey protein concentrate) were studied by means of electron spectroscopy for chemical analysis (ESCA).

TL;DR: In this article, the adhesion forces of Si3N4 tips to the surfaces of Gram-negative bacterial strains possessing different lipopolysacharides (LPS) (i.e., Pseudomonas aeruginosa PAO1 and its isogenic mutants) was investigated without the use of surface modifying or bonding agents to adhere cells to the filter.

TL;DR: In this paper, the apparent potential of raw milk fat globules, either natural or mechanically damaged by homogenization or shear, was measured by laser Doppler electrophoresis.

TL;DR: An overview of the application of atomic force microscopy for the investigation of bacterial biofilms focusing on specific studies related to metallic surfaces such as stainless steel and copper alloys in freshwater and marine environments is provided in this article.

TL;DR: In this paper, streaming potential and current measurements on oxide layers on Ti6Al4V and Ti, carried out using a micro-slit electrokinetic set-up (MES) and a commercial EKA, PAAR, were investigated.

TL;DR: In this article, the Newman strain of Staphylococcus aureus adhesion on self-assembled monolayers (SAMs) formed by the adsorption of alkanethiols on transparent gold films has been studied in real time under well-defined flow conditions using a radial flow chamber and an automated videomicroscopy system.

TL;DR: In this paper, the effects of both polymeric composition and technological parameters on release profiles of 5-fluorouracil (5-fluoro-2,4-pyrimidinedione) were investigated.

TL;DR: Experiments with bilayers consisting of DOTAP and dioleoylphosphatidylserine (DOPS) show that the break-through forces behave in the predicted way.

TL;DR: The model of zero-order release with first-order re-encapsulation appears to represent data from this polymeric system better than other models and has possible applications in site-specific chemotherapy.

TL;DR: At atomic force microscopy (AFM) is used to probe the nanoscale morphology and molecular interactions of polystyrene substrata after adhesion of the Gram-negative bacterium Azospirillum brasilense, and the correlation between the AFM data and the cell adhesion behaviour provides evidence for the involvement of proteinaceous EPS in the adhesion

TL;DR: In this article, a modified Derjaguin-Landau-Verwey-Overbeek (DLVO) theory was used to calculate the total intersurface potential energy as a function of separation distance between bacterial and sediment surfaces.

TL;DR: In this paper, local anodic oxidation (LAO) was used to create nanostructures of sizes comparable to protein dimensions on Si and Ti surfaces by using the atomic force microscope (AFM).

TL;DR: In this article, three sucrose oligoesters having palmitic acid, stearic acid and lauric acid moieties were added to PKO as additives, in an attempt to accelerate the crystallisation of PKO in the O/W emulsions that was suppressed due to dispersion of the oil phase into many droplets.

TL;DR: In this paper, the adsorption of the globular protein β-lactoglobulin on hydrophilic and hydrophobic surfaces has been investigated using spectroscopic ellipsometry and Fourier transform infrared spectroscopy in the attenuated total reflection mode.

TL;DR: A growing body of evidence shows that nonlamellar-forming membrane lipids play essential roles in many aspects of membrance functioning, including a proper cell division, and an efficient translocation of proteins across the plasma membrane of E. coli cells.

TL;DR: Rod-like calcium hydroxyapatites (Hap) with micrometer size were produced by supplying Ca(CH 3 COO) 2 and NH 4 H 2 PO 4 solutions into a CH 3 COONH 4 solution by varying their feeding rate at 85±5 °C and pH 7.4±0.2 under nitrogen atmosphere and vigorously stirring as discussed by the authors.

TL;DR: As the first preclinical trial of nisin-treated implantable materials, this study represents an important first step for developing the potentially broad use of protein antimicrobial films on implantable medical devices.

TL;DR: It is shown that force spectroscopy reveals information about kinetic rates, affinities, and the dynamic structure of the binding pocket, which shows great potential for investigating the molecular dynamics of ligand–receptor binding and the epitope mapping of receptors.

TL;DR: In this article, a two-step procedure based on low pressure plasma treatment followed by a grafting reaction was used to obtain a durable and homogeneous surface functionalization of poly(tetrafluoroethylene) (PTFE).

TL;DR: In this paper, two strains (an environmental strain and a reference one coming from a national culture collection) of Yersinia ruckeri, a fish pathogenic bacterium, are characterised according to the ability to adhere on wood, concrete, polyvinylchloride (PVC) and fibreglass, four materials commonly found in fish farms.

TL;DR: The initial hour of attachment of Pseudomonas aeruginosa to ZnSe and to TiO2 from solutions of different ionic strength and pH was studied using in situ attenuated total reflection infrared (ATR-IR) spectroscopy.

TL;DR: This article reviews the present knowledge on the extracellular lipolytic enzymes LipA and LipB from Bacillus subtilis and building a three-dimensional structural model of LipB using the atomic coordinates of the LipA crystal structure, which was solved recently, revealed that 43 out of 45 residues, which are different from LipA, were located on the surface oflipB.

TL;DR: In this paper, a model for the building up of the BSA layer is proposed, which accounts for these data, and the authors make clear a higher reactivity of chromium towards the protein compared to stainless steel.

TL;DR: The results showed that separation work is a good measure of specific interactions between FN and the cell surface, and the system developed here is a promising quantitative method to evaluate cell adhesion force.

TL;DR: In this paper, a pure preparation of human salivary MUC5B was used together with a commercial one of bovine submaxillary mucin (BSM) to investigate the behavior of Salivary mucins at solid surfaces with different wettabilities, as well as the influence of electrolyte on the adsorption behaviour.

TL;DR: In this article, the electrophoretic mobility of a number of environmentally important microorganisms in phosphate buffers was measured and the effect of pH, ionic strength, and cation type (valence) and concentration on the EPM was investigated.

TL;DR: In this paper, a microwave-UV light sterilization system was constructed and investigated its effect on the generation of active oxygen species such as hydroxyl radical or superoxide in the solution.

TL;DR: The bioconjugate material exhibited an increased half-life of the enzyme at 60 °C and pH 9 relative to that of the free enzyme in solution, thus highlighting the role of colloidal gold particles in stabilizing the enzyme molecules.

TL;DR: In this paper, the role of ionic strength and pH in determining the surface properties and hence adsorption of the bacterium Bacillus polymyxa to hematite and quartz has been studied in detail.