Journal ArticleDOI
rab5 controls early endosome fusion in vitro.
Reads0
Chats0
TLDR
In vitro findings strongly suggest that rab5 is involved in the process of early endosome fusion, and the inhibition mediated by anti-rab5 antibodies could be overcome by complementing the assay with the cytosol containing wild-type rab5, but not with the same cytOSol depleted of rab5.About:
This article is published in Cell.The article was published on 1991-03-08. It has received 1080 citations till now. The article focuses on the topics: EEA1 & Endosome.read more
Citations
More filters
Journal ArticleDOI
Rab proteins as membrane organizers
Marino Zerial,Heidi M. McBride +1 more
TL;DR: Cellular organelles in the exocytic and endocytic pathways have a distinctive spatial distribution and communicate through an elaborate system of vesiculo-tubular transport.
Journal ArticleDOI
Rab GTPases as coordinators of vesicle traffic.
TL;DR: Crosstalk between multiple Rab GTPases through shared effectors, or through effectors that recruit selective Rab activators, ensures the spatiotemporal regulation of vesicle traffic.
Journal ArticleDOI
Mechanisms of phagocytosis in macrophages
Alan Aderem,David M. Underhill +1 more
TL;DR: Macrophages also play an important role in the recognition and clearance of apoptotic cells; a notable feature of this process is the absence of an inflammatory response.
Journal ArticleDOI
Small GTP-Binding Proteins
TL;DR: In this review, functions of small G proteins and their modes of activation and action are described.
Journal ArticleDOI
The synaptic vesicle cycle: a cascade of protein–protein interactions
TL;DR: A convergence of results now allows formulation of molecular models for key steps of the synaptic vesicle cycle, which may form the basis for a mechanistic understanding of higher neural function.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
TL;DR: The detection of murine leukemia virus antigens in complex cellular lysates was used to demonstrate the efficacy of this electrophoretic transfer technique.
Journal ArticleDOI
Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase
TL;DR: The vaccinia/T7 hybrid virus forms the basis of a simple, rapid, widely applicable, and efficient mammalian expression system.
Book ChapterDOI
Polyacrylamide Gel Electrophoresis of Viral Proteins
TL;DR: This chapter describes polyacrylamide gel electrophoresis of viral proteins in gels, which are extensively used at the present time and are more homogeneous and stable than other commonly used materials.
Journal ArticleDOI
Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments.
TL;DR: Findings provide evidence that members of the YPT1/SEC4 subfamily of GTP binding proteins are localized to specific exocytic and endocytic subcompartments in mammalian cells.