Journal ArticleDOI
Rapid isolation of DNA probes within specific chromosome regions by interspersed repetitive sequence polymerase chain reaction.
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TLDR
The results demonstrate that primers can be constructed to a variety of interspersed repetitive sequences (IRS) and used individually or in combination for the rapid isolation of DNA fragments from defined chromosomal regions by IRS-PCR.About:
This article is published in Genomics.The article was published on 1990-03-01. It has received 181 citations till now. The article focuses on the topics: Interspersed repeat & Interspersed Repetitive Sequences.read more
Citations
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Journal ArticleDOI
Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Journal ArticleDOI
Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
Håkan Telenius,Nigel P. Carter,C. E. Bebb,M. Nordenskjold,Bruce A.J. Ponder,Alan Tunnacliffe +5 more
TL;DR: DOP-PCR represents a rapid, efficient, and species-independent technique for general DNA amplification, and has advantages over interspersed repetitive sequence PCR (IRS- PCR), which relies on the appropriate positioning of species-specific repeat elements.
Journal ArticleDOI
Recent advances in the polymerase chain reaction
TL;DR: Progresses ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences and some recent developments in instrumentation, methodology, and applications of the PCR are presented.
Journal ArticleDOI
Alu repeats and human disease.
TL;DR: Between these different mechanisms, Alu elements have not only contributed a great deal to the evolution of the genome but also continue to contribute to a significant portion of human genetic diseases.
Journal ArticleDOI
Construction of YAC-based mammalian artificial chromosomes.
Masashi Ikeno,Brenda Grimes,Tuneko Okazaki,Tuneko Okazaki,Megumi Nakano,Kaori Saitoh,Harumi Hoshino,Niolette I. McGill,Howard J. Cooke,Hiroshi Masumoto +9 more
TL;DR: Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism, which suggests they have potential as vectors for introducing genes into mammals.
References
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Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
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Hypervariable 'minisatellite' regions in human DNA.
TL;DR: A probe based on a tandem-repeat of the core sequence can detect many highly variable loci simultaneously and can provide an individual-specific DNA ‘fingerprint’ of general use in human genetic analysis.
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Variable number of tandem repeat (VNTR) markers for human gene mapping
Yusuke Nakamura,Mark Leppert,Peter O'Connell,Roger K. Wolff,Tom Holm,Melanie Culver,Cindy Martin,Esther Fujimoto,Mark Hoff,Erika Kumlin,Ray White +10 more
TL;DR: Ten oligomeric sequences derived from the tandem repeat regions of the myoglobin gene, the zeta-globin pseudogene, the insulin gene, and the X-gene region of hepatitis B virus were used to develop a series of single-copy probes that revealed new, highly polymorphic genetic loci whose allele sizes reflected variation in the number of tandem repeats.
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A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes
TL;DR: A huge number of stretches of dT-dG alternating sequence, a sequence that has been shown to adopt the Z-DNA conformation under some conditions, are found in eukaryotic genomes, indicating extraordinary evolutionary conservation.