Journal ArticleDOI
Rapid scanning fluorescence spectroscopy using an acousto‐optic tunable filter
Ira Kurtz,R. Dwelle,P. Katzka +2 more
TLDR
In this article, a new method for rapidly acquiring fluorescence spectra from optical probes using an acousto-optic tunable filter (AOTF) was developed, which consists of a piezoelectric transducer bonded to a birefringent crystal.Abstract:
A new method for rapidly acquiring fluorescence spectra from optical probes using an acousto‐optic tunable filter (AOTF) has been developed. The AOTF consists of a piezoelectric transducer bonded to a birefringent crystal. Acoustic waves are generated in the crystal by an applied radio frequency. For a given radio frequency, only a narrow band of optical frequencies will be diffracted. Unlike normal Bragg diffraction which can only be used with collimated light, the AOTF has a large angular aperture (up to 28 deg) and can therefore be coupled to an uncollimated broadband white light source, photomultiplier tube, or camera. Using the device and a xenon–mercury arc lamp, excitation and emission spectra of the fluorescent pH probe BCECF were acquired in 17 ms (spectral resolution=4 nm). Rapid wavelength switching or modulation of selected wavelengths was achieved at ∼105 Hz. The device is also capable of spectral imaging with a spatial resolution of greater than 100 lines/mm.read more
Citations
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Journal ArticleDOI
Imaging Spectrometers for Fluorescence and Raman Microscopy: Acousto-Optic and Liquid Crystal Tunable Filters:
TL;DR: In this article, acoustic tunable filters (AOTF) and liquid crystal tunable filter (LCTF) are evaluated for their suitability as fluorescence microscopy imaging spectrometers.
Patent
Spectroscopic imaging device employing imaging quality spectral filters
TL;DR: In this article, an acousto-optic tunable filter (AOTF) or an interferometer, and a focal plane array detector are used as the imaging detector in both cases.
Journal ArticleDOI
Practical design criteria for a dynamic ratio imaging system.
TL;DR: Goals for Ca2(+)-imaging include real-time ratioing, psychophysically effective display formats, ease of experiment annotation, mass storage of image sequences, automated extraction of time courses and population statistics, aids to presenting images for seminars and publications, and program modifiability.
Patent
Method for simultaneously measuring the spectral intensity as a function of wavelength of all the pixels of a two dimensional scene
TL;DR: In this article, a method of analyzing an optical image of a scene to determine the spectral intensity of each pixel of the scene, which includes collecting incident light from the scene and passing the light through an interferometer which outputs modulated light corresponding to a predetermined set of linear combinations of the intensity of the light emitted from each pixel, focusing the light outputted from the inter-ferometer on a detector array, and processing the output of the detector array to determine each pixel thereof, is presented.
Journal ArticleDOI
Multivariate optical computation for predictive spectroscopy.
TL;DR: It is concluded that many types of predictive measurements based on use of regression vectors and linear mathematics can be performed more rapidly, more effectly, and at considerably lower cost by the proposed optical computation method than by traditional dispersive or interferometric instrumentation.
References
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Journal ArticleDOI
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.
Book
Principles of Optics
TL;DR: In this paper, the authors discuss various topics about optics, such as geometrical theories, image forming instruments, and optics of metals and crystals, including interference, interferometers, and diffraction.
Principles of Optics
TL;DR: In this article, the authors discuss various topics about optics, such as geometrical theories, image forming instruments, and optics of metals and crystals, including interference, interferometers, and diffraction.
Journal ArticleDOI
Cytoplasmic pH and free Mg2+ in lymphocytes.
TL;DR: Measurements have been made of cytoplasmic pH, (pHi) and free Mg2+ concentration, ( [Mg2 +]i), in pig and mouse lymphocytes, and Mitogenic concentrations of concanavalin A (Con A) had no measurable effect on pH in the first hour.
Journal ArticleDOI
Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2
TL;DR: The observed regulation of intranuclear Ca2+ in these contractile cells may be seen as a way to prevent fluctuation in Ca2-linked nuclear processes during the rise in cytoplasmic [Ca2+] which triggers contraction.