Rational Design of Programmable Monodisperse Semi-Synthetic Protein Nanomaterials Containing Engineered Disulfide Functionality**
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In this paper, a new chemical methodology for the construction of redox-sensitive protein assemblies using monodisperse facially amphiphilic protein-dendron bioconjugates is presented.Abstract:
The reversible nature of disulfide functionality has been exploited to design intelligent materials such as nanocapsules, micelles, vesicles, inorganic nanoparticles, peptide and nucleic acid nanodevices. Herein, we report a new chemical methodology for the construction redox-sensitive protein assemblies using monodisperse facially amphiphilic protein-dendron bioconjugates. The disulfide functionality is strategically placed between the dendron and protein domains. The custom designed bioconjugates self-assembled into nanoscopic objects of a defined size dictated by the nature of dendron domain. The stimuli-responsive behavior of the protein assemblies is demonstrated using a suitable redox trigger.read more
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Rational Design of Self-assembling Artificial Proteins Utilizing a Micelle-Assisted Protein Labeling Technology (MAPLabTech): Testing the Scope
TL;DR: In this article, the synthesis of self-assembling artificial proteins (SAPs) using micelle-assisted protein labeling technology (MAPLabTech) has been described by developing a chemical method for site-specific labeling of a surfaceexposed cysteine residue of globular proteins.
Journal ArticleDOI
Rational Design of Self‐Assembling Artificial Proteins Utilizing a Micelle‐Assisted Protein Labeling Technology (MAPLabTech): Testing the Scope **
Mullapudi Mohan Reddy,Pavankumar Janardhan Bhandari,Kshitish Chandra Hati,Britto S. Sandanaraj +3 more
TL;DR: The synthesis of novel SAPs are described by developing a chemical method for site‐specific labeling of a surface‐exposed cysteine residue of globular proteins and their systematic self‐assembly and disassembly studies using size‐exclusion chromatography are disclosed.
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