Raw Cow Milk Bacterial Consortium as Bioindicator of Circulating Anti-Microbial Resistance (AMR).
Cristian Piras,Viviana Greco,Viviana Greco,Enrico Gugliandolo,Alessio Soggiu,Bruno Tilocca,Luigi Bonizzi,Alfonso Zecconi,Rainer Cramer,Domenico Britti,Andrea Urbani,Andrea Urbani,Paola Roncada +12 more
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TLDR
(meta)proteomics analysis of bacterial compartment of raw milk is applied to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition.Abstract:
The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.read more
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References
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Occurrence of β-Lactamase-Producing Gram-Negative Bacterial Isolates in Water Sources in Cali City, Colombia
TL;DR: The results indicate that β-lactamase-producing Gram-negative bacteria are widespread in the environment in the aquatic ecosystem of Cali city, and this river can be considered as a medium for the spread of bacterial antibiotic resistance genes.
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Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Adds Enhanced Functionalities to MALDI MS Profiling for Disease Diagnostics.
TL;DR: The potential of this MALDI MS profiling method is demonstrated on the detection of dairy cow mastitis, which is a substantial economic burden on the dairy industry with losses of hundreds of dollars per diseased cow per year, as well as leading to the use of large quantities of antibiotics, adding to the proliferation of antimicrobial resistance.
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Exploring the HeLa Dark Mitochondrial Proteome.
Federica Marini,Federica Marini,Victor Corasolla Carregari,Victor Corasolla Carregari,Viviana Greco,Viviana Greco,Maurizio Ronci,Federica Iavarone,Federica Iavarone,Silvia Persichilli,Massimo Castagnola,Andrea Urbani,Luisa Pieroni +12 more
TL;DR: This work implemented an experimental workflow, combining classical biochemical enrichments and mass spectrometry, to pursue a much deeper definition of mitochondrial proteome and possibly mine mitochondrial uncharacterized dark proteins.
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Short communication: Improved method for centrifugal recovery of bacteria from raw milk applied to sensitive real-time quantitative PCR detection of Salmonella spp.
TL;DR: This is the first report of an effective method for achieving high centrifugal recovery of bacteria from raw milk without impairing bacterial viability and a simple, rapid agitation treatment was developed that disrupted bacteria binding to the cream layer and provided more than 95% recovery without affecting bacteria viability.
Journal ArticleDOI
Rapid liquid AP-MALDI MS profiling of lipids and proteins from goat and sheep milk for speciation and colostrum analysis
Cristian Piras,Carlotta Ceniti,Evita Hartmane,Nicola Costanzo,Valeria Maria Morittu,Paola Roncada,Domenico Britti,Rainer Cramer +7 more
TL;DR: The data show that substantial changes in the lipid profile can account for the accurate classification of colostrum collected at the early and late time points, and allow unequivocal speciation analysis.
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