Raw Cow Milk Bacterial Consortium as Bioindicator of Circulating Anti-Microbial Resistance (AMR).
Cristian Piras,Viviana Greco,Viviana Greco,Enrico Gugliandolo,Alessio Soggiu,Bruno Tilocca,Luigi Bonizzi,Alfonso Zecconi,Rainer Cramer,Domenico Britti,Andrea Urbani,Andrea Urbani,Paola Roncada +12 more
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TLDR
(meta)proteomics analysis of bacterial compartment of raw milk is applied to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition.Abstract:
The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.read more
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