Improving reproducibility in animal research by splitting the study population into several ‘mini-experiments’
Vanessa Tabea von Kortzfleisch,Natasha A. Karp,Rupert Palme,Sylvia Kaiser,Norbert Sachser,S. Helene Richter +5 more
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It is shown that the use of such a ‘mini-experiment’ design improved the reproducibility and accurate detection of exemplary treatment effects in about half of all investigated strain comparisons.Abstract:
In light of the hotly discussed ‘reproducibility crisis’, a rethinking of current methodologies appears essential. Implementing multi-laboratory designs has been shown to enhance the external validity and hence the reproducibility of findings from animal research. We here aimed at proposing a new experimental strategy that transfers this logic into a single-laboratory setting. We systematically introduced heterogeneity into our study population by splitting an experiment into several ‘mini-experiments’ spread over different time points a few weeks apart. We hypothesised to observe improved reproducibility in such a ‘mini-experiment’ design in comparison to a conventionally standardised design, according to which all animals are tested at one specific point in time. By comparing both designs across independent replicates, we could indeed show that the use of such a ‘mini-experiment’ design improved the reproducibility and accurate detection of exemplary treatment effects (behavioural and physiological differences between four mouse strains) in about half of all investigated strain comparisons. Thus, we successfully implemented and empirically validated an easy-to-handle strategy to tackle poor reproducibility in single-laboratory studies. Since other experiments within different life science disciplines share the main characteristics with the investigation reported here, these studies are likely to also benefit from this approach.read more
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Potential of microalgae as a sustainable feed ingredient for aquaculture
Senthil Nagappan,Probir Das,Mohammad AbdulQuadir,Mahmoud Thaher,Shoyeb Khan,Chandan Mahata,Hareb Mohammed S.J. Al-Jabri,Ann Kristin Vatland,Gopalakrishnan Kumar +8 more
TL;DR: In this paper, the authors explore the potential of producing microalgae biomass as an ingredient of aquaculture feed, and show that the cell metabolites of these microorganisms contain a blend of essential amino acids, healthy triglycerides as fat, vitamins, and pigments.
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Fusobacterium nucleatum is associated with worse prognosis in Lauren's diffuse type gastric cancer patients.
Ellen Teresa Boehm,Cosima Thon,Juozas Kupcinskas,Ruta Steponaitiene,Jurgita Skieceviciene,Ali Canbay,Peter Malfertheiner,Alexander Link +7 more
TL;DR: Novel evidence is provided for clinical relevance of F. nucleatum in GC by showing an association between F.ucleatum positivity with worse prognosis of patients with Laurens’s diffuse type gastric cancer.
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Close to the edge: Heterochromatin at the nucleolar and nuclear peripheries.
Aizhan Bizhanova,Paul D. Kaufman +1 more
TL;DR: Pioneering studies of NADs and LADs are summarized, more recent advances in the understanding of cis/trans-acting factors that mediate these localizations are discussed, and the functional significance of these associations are discussed.
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Autism spectrum disorder and pupillometry: A systematic review and meta-analysis.
TL;DR: Pupillometry reveals differences between people with and without ASD, but the exact meaning of these differences remains unknown, and future studies should align research designs and investigate a possible effect of maturation.
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Use of dried blood spot samples for SARS-CoV-2 antibody detection using the Roche Elecsys ® high throughput immunoassay.
Ranya Mulchandani,Ben Brown,Tim Brooks,Amanda Semper,Nicholas Machin,Ezra Linley,Ray Borrow,David H. Wyllie +7 more
TL;DR: In this article, the authors compared antibody detection in DBS eluates using the Roche Elecsys ® immunoassay with antibody detection using paired plasma samples, using the same assay, aiming to compare the applicability of DBS based approaches for SARS-CoV-2 antibody detection.
References
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TL;DR: A large-scale assessment suggests that experimental reproducibility in psychology leaves a lot to be desired, and correlational tests suggest that replication success was better predicted by the strength of original evidence than by characteristics of the original and replication teams.
Book
The principles of humane experimental technique
W. M. S. Russell,R. L. Burch +1 more
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Animal research: reporting in vivo experiments: the ARRIVE guidelines
TL;DR: An accurate summary of the background, research objectives, including details of the species or strain of animal used, key methods, principal findings and conclusions of the study is provided.