Raw Cow Milk Bacterial Consortium as Bioindicator of Circulating Anti-Microbial Resistance (AMR).
Cristian Piras,Viviana Greco,Viviana Greco,Enrico Gugliandolo,Alessio Soggiu,Bruno Tilocca,Luigi Bonizzi,Alfonso Zecconi,Rainer Cramer,Domenico Britti,Andrea Urbani,Andrea Urbani,Paola Roncada +12 more
Reads0
Chats0
TLDR
(meta)proteomics analysis of bacterial compartment of raw milk is applied to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition.Abstract:
The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.read more
Citations
More filters
Journal ArticleDOI
Unravelling direct and indirect contact patterns between duck farms in France and their association with the 2016–2017 epidemic of Highly Pathogenic Avian Influenza (H5N8)
Billy Bauzile,G. Sicard,Claire Guinat,Claire Guinat,Mathieu Andraud,Nicolas Rose,P. Hammami,Benoit Durand,Mathilde Paul,Timothée Vergne +9 more
TL;DR: In this article, the authors compared direct and indirect contact patterns between farms and evaluate how these patterns were associated with the French 2016-2017 epidemic of highly pathogenic avian influenza H5N8.
Journal ArticleDOI
Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning
Marina López-Arjona,Eva Mainau,Elena Navarro,María Dolores Contreras-Aguilar,Damián Escribano,Sandra V. Mateo,Xavier Manteca,José J. Cerón,Silvia Martínez-Subiela +8 more
TL;DR: In this paper, two new assays (one using a monoclonal antibody and the other using a polyclonal antibody) for the measurement of oxytocin in bovine saliva were developed and validated.
Journal ArticleDOI
Tannins in the diet for lactating goats from different genetic groups in the Brazilian semiarid: Nitrogen, energy and water balance
Ana Paula Ribeiro da Silva,Aline Silva de Sant’ana,Sheyla Priscila Oliveira do Nascimento,Steyce Neves Barbosa,Ana Laura Alencar Miranda,Fabrina de Sousa Luna,Glayciane Costa Gois,Salete Alves de Moraes,Rafael Torres de Souza Rodrigues,Daniel Ribeiro Menezes +9 more
TL;DR: In this article, the authors evaluated intake, milk production, nitrogen balance, energy balance, and water balance of lactating goats given diets with and without condensed tannin, with an average age of 5 years and 30 days of lactation, and the experimental period lasted 30 days, with 25 days for adaptation of animals and 5 days for data collection.
Book ChapterDOI
History and Present Situations of Robotic Harvesting Technology: A Review
Jizhan Liu,Zhiguo Li,Pingping Li +2 more
TL;DR: According to the statistics, the global production of total fruits and vegetables in 2019 reached 8.83 × 108t and 11.30× 108t, respectively as mentioned in this paper, respectively.
Journal ArticleDOI
Absence of Hemolivia mauritanica (Apicomplexa: Haemogregarinidae) in natural populations of Testudo graeca in Morocco.
El-Mustapha Laghzaoui,Dimitra Sergiadou,Ana Perera,D. James Harris,Abdelaziz Abbad,El Hassan El Mouden +5 more
TL;DR: The results support and confirm the finding of studies previously conducted in other populations of Morocco and indicate that H. mauritanica has a narrower distribution range than its tick vector.
References
More filters
Journal ArticleDOI
Universal sample preparation method for proteome analysis
TL;DR: A method is described, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics and allows single-run analyses of organelles and an unprecedented depth of proteome coverage.
Journal ArticleDOI
CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database
Baofeng Jia,Amogelang R. Raphenya,Brian Alcock,Nicholas Waglechner,Peiyao Guo,Kara K. Tsang,Briony A. Lago,Biren M. Dave,Sheldon K. Pereira,Arjun N. Sharma,Sachin Doshi,Mélanie Courtot,Raymond Lo,Laura E. Williams,Jonathan G. Frye,Tariq Elsayegh,Daim Sardar,Erin L. Westman,Andrew C. Pawlowski,Timothy A. Johnson,Fiona S. L. Brinkman,Gerard D. Wright,Andrew G. McArthur +22 more
TL;DR: The Comprehensive Antibiotic Resistance Database (CARD) is a manually curated resource containing high quality reference data on the molecular basis of antimicrobial resistance (AMR), with an emphasis on the genes, proteins and mutations involved in AMR.
Journal ArticleDOI
CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database
Brian Alcock,Amogelang R. Raphenya,Tammy T. Y. Lau,Kara K. Tsang,Mégane Bouchard,Arman Edalatmand,William Huynh,Anna-Lisa V. Nguyen,Annie A. Cheng,Sihan Liu,Sally Y. Min,Anatoly Miroshnichenko,Hiu-Ki R Tran,Rafik El Werfalli,Jalees A. Nasir,Martins Oloni,David Speicher,Alexandra Florescu,Bhavya Singh,Mateusz Faltyn,Anastasia Hernández-Koutoucheva,Arjun N. Sharma,Emily Bordeleau,Andrew C. Pawlowski,Haley L. Zubyk,Damion M. Dooley,Emma Griffiths,Finlay Maguire,Geoffrey L. Winsor,Robert G. Beiko,Fiona S. L. Brinkman,William W. L. Hsiao,William W. L. Hsiao,Gary Van Domselaar,Gary Van Domselaar,Andrew G. McArthur +35 more
TL;DR: A new Resistomes & Variants module provides analysis and statistical summary of in silico predicted resistance variants from 82 pathogens and over 100 000 genomes, able to summarize predicted resistance using the information included in CARD, identify trends in AMR mobility and determine previously undescribed and novel resistance variants.
Journal ArticleDOI
Antibiotic Resistance Is Prevalent in an Isolated Cave Microbiome
Kirandeep Bhullar,Nicholas Waglechner,Andrew C. Pawlowski,Kalinka Koteva,Eric D. Banks,Michael D. Johnston,Hazel A. Barton,Gerard D. Wright +7 more
TL;DR: A screen of a sample of the culturable microbiome of Lechuguilla Cave, New Mexico, in a region of the cave that has been isolated for over 4 million years reported that, like surface microbes, these bacteria were highly resistant to antibiotics; some strains were resistant to 14 different commercially available antibiotics.
Journal ArticleDOI
Intrinsic antibiotic resistance: Mechanisms, origins, challenges and solutions
Georgina Cox,Gerard D. Wright +1 more
TL;DR: The possibility of inhibiting elements comprising the intrinsic resistome in bacterial pathogens offers the promise for repurposing existing antibiotics against intrinsically resistant bacteria.
Related Papers (5)
Genetic Analysis of Human Norovirus Strains in Japan in 2016-2017.
Koo Nagasawa,Yuki Matsushima,Takumi Motoya,Fuminori Mizukoshi,Yo Ueki,Naomi Sakon,Koichi Murakami,Tomomi Shimizu,Nobuhiko Okabe,Noriko Nagata,Komei Shirabe,Hiroto Shinomiya,Wataru Suzuki,Makoto Kuroda,Tsuyoshi Sekizuka,Yoshiyuki Suzuki,Akihide Ryo,Kiyotaka Fujita,Kazunori Oishi,Kazuhiko Katayama,Hirokazu Kimura,Hirokazu Kimura +21 more