Journal ArticleDOI
Reactant Minimization for Sample Preparation on Microfluidic Biochips With Various Mixing Models
TLDR
This paper proposes the first sample preparation method dedicated to microfluidic biochips with various mixing models, named tree pruning and grafting (TPG) algorithm, which starts with a dilution tree created by regarding the (1:1) mixing model only, and applies TPG through a bottom-up dynamic programming strategy to obtain a solution with minimal reactant consumption.Abstract:
Sample preparation is one of the essential processes for most on-chip biochemical applications. During this process, raw reactants are diluted to specific concentration values. Current sample preparation algorithms are generally created for digital microfluidic biochips with the (1:1) mixing model. For other biochip architectures supporting multiple mixing models, such as flow-based microfluidic biochips, there is still no dedicated solution yet. Hence, in this paper, we propose the first sample preparation method dedicated to microfluidic biochips with various mixing models, named tree pruning and grafting (TPG) algorithm. It starts with a dilution tree created by regarding the (1:1) mixing model only, and then applies TPG through a bottom-up dynamic programming strategy to obtain a solution with minimal reactant consumption. Experimental results show that our algorithm can save reactant amount by up to 69% against the well-known bit-scanning method on a biochip with a four-segment mixer. Even compared with the state-of-the-art reactant minimization algorithm, it still achieves a reactant reduction of 37%. Therefore, it is convincing that the TPG algorithm is a promising sample preparation solution for biochip architectures that support various mixing models.read more
Citations
More filters
Journal ArticleDOI
Dilution and Mixing Algorithms for Flow-Based Microfluidic Biochips
TL;DR: The proposed satisfiability-based dilution algorithm outperforms existing dilution algorithms in terms of mixing steps and waste production, and compares favorably with respect to reagent-usage (cost) when 4- and 8-segment rotary mixers are used.
Proceedings ArticleDOI
LUTOSAP: Lookup Table Based Online Sample Preparation in Microfluidic Biochips
TL;DR: LUTOSAP enables fast query response for online sample preparation requirements with the solution where the weighted sum of sample consumption, buffer consumption, and the number of mix-split operations is optimized.
Journal ArticleDOI
Design automation for continuous-flow microfluidic biochips: A comprehensive review
Genggeng Liu,Antonio Ventosa,Hongbin Huang,Zhisheng Chen,Hongxing Lin,Hui Liu,Xing Huang,Wenzhong Guo +7 more
TL;DR: A comprehensive review of recent advances in the design automation of CFMBs, including CAD techniques for architecture synthesis, volume assignment and sample preparation, testing, fault-tolerant design, and washing are presented.
Journal ArticleDOI
Storage-Aware Algorithms for Dilution and Mixture Preparation With Flow-Based Lab-on-Chip
TL;DR: This paper proposes new algorithms for dilution and mixing with continuous-flow-based LoC architectures that explicitly take care of storage constraints while optimizing reactant-cost and time of sample preparation.
Journal ArticleDOI
Design automation for continuous-flow microfluidic biochips: A comprehensive review
TL;DR: A comprehensive review of recent advances in the design automation of CFMBs, including CAD techniques for architecture synthesis, volume assignment and sample preparation, testing, fault-tolerant design, and washing, is presented in this paper .
References
More filters
Journal ArticleDOI
The origins and the future of microfluidics
TL;DR: The manipulation of fluids in channels with dimensions of tens of micrometres — microfluidics — has emerged as a distinct new field that has the potential to influence subject areas from chemical synthesis and biological analysis to optics and information technology.
PatentDOI
Microfluidic large scale integration
TL;DR: The fluidic multiplexor as discussed by the authors is a combinatorial array of binary valve patterns that exponentially increases the processing power of a network by allowing complex fluid manipulations with a minimal number of inputs.
Journal ArticleDOI
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications
TL;DR: This critical review summarizes developments in microfluidic platforms that enable the miniaturization, integration, automation and parallelization of (bio-)chemical assays and attempts to provide a selection scheme based on key requirements of different applications and market segments.
Microfluidic Large Scale Integration
TL;DR: High-density microfluidic chips that contain plumbing networks with thousands of micromechanical valves and hundreds of individually addressable chambers are developed to construct the microfluidity analog of a comparator array and a microfluidsic memory storage device whose behavior resembles random-access memory.
Journal ArticleDOI
The past, present and potential for microfluidic reactor technology in chemical synthesis
TL;DR: The utility of microfluidic reactor technology as a tool in chemical synthesis in both academic research and industrial applications and the current roadblocks hindering the widespread use are assessed.