Reciprocal tripartite interactions between the Aedes aegypti midgut microbiota, innate immune system and dengue virus influences vector competence.
Jose Luis Ramirez,Jayme A. Souza-Neto,Rolando Torres Cosme,Jose R. Rovira,Alma Ortiz,Juan Miguel Pascale,George Dimopoulos +6 more
TLDR
It is shown that the mosquito's microbiota influences dengue virus infection of the mosquito, which in turn activates its antibacterial responses, which influences the microbial load of the mosquitoes midgut.Abstract:
Dengue virus is one of the most important arboviral pathogens and the causative agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. It is transmitted between humans by the mosquitoes Aedes aegypti and Aedes albopictus, and at least 2.5 billion people are at daily risk of infection. During their lifecycle, mosquitoes are exposed to a variety of microbes, some of which are needed for their successful development into adulthood. However, recent studies have suggested that the adult mosquito's midgut microflora is critical in influencing the transmission of human pathogens. In this study we assessed the reciprocal interactions between the mosquito's midgut microbiota and dengue virus infection that are, to a large extent, mediated by the mosquito's innate immune system. We observed a marked decrease in susceptibility to dengue virus infection when mosquitoes harbored certain field-derived bacterial isolates in their midgut. Transcript abundance analysis of selected antimicrobial peptide genes suggested that the mosquito's microbiota elicits a basal immune activity that appears to act against dengue virus infection. Conversely, the elicitation of the mosquito immune response by dengue virus infection itself influences the microbial load of the mosquito midgut. In sum, we show that the mosquito's microbiota influences dengue virus infection of the mosquito, which in turn activates its antibacterial responses.read more
Citations
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The invasive mosquito species Aedes albopictus: current knowledge and future perspectives
TL;DR: Details of population genetics and structure will allow following, and possibly predicting, the geographical and temporal dynamics of its expansion, and will inform the practical operations of control programs.
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Diversity and function of bacterial microbiota in the mosquito holobiont.
TL;DR: A review of the diversity and functions of mosquito-associated bacteria across multiple variation factors, emphasizing recent findings, is presented.
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Global temperature constraints on Aedes aegypti and Ae. albopictus persistence and competence for dengue virus transmission
Oliver J. Brady,Nick Golding,David M. Pigott,Moritz U. G. Kraemer,Jane P. Messina,Robert C. Reiner,Robert C. Reiner,Thomas W. Scott,Thomas W. Scott,David L. Smith,David L. Smith,David L. Smith,Peter W. Gething,Simon I. Hay,Simon I. Hay +14 more
TL;DR: An existing modelling framework is expanded with new temperature-based relationships to model an index proportional to the basic reproductive number of the dengue virus, which predicted areas where temperature is not expected to permit transmission and/or Aedes persistence throughout the year and suggests Ae.
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Impact of environment on mosquito response to pyrethroid insecticides: Facts, evidences and prospects
TL;DR: Current knowledge on pyrethroid resistance mechanisms in mosquitoes is updated and available data on the impact of the environment on mosquito response to pyrethroids, including key environmental factors, such as the presence of urban or agricultural pollutants and biotic interactions between mosquitoes and their microbiome are discussed, and research perspectives to fill in knowledge gaps are suggested.
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Chromobacterium Csp_P Reduces Malaria and Dengue Infection in Vector Mosquitoes and Has Entomopathogenic and In Vitro Anti-pathogen Activities
Jose Luis Ramirez,Sarah M. Short,Ana C. Bahia,Raúl G. Saraiva,Yuemei Dong,Seokyoung Kang,Abhai K. Tripathi,Godfree Mlambo,George Dimopoulos +8 more
TL;DR: This bacterium isolated from the midgut of field-caught Aedes aegypti exerts in vitro anti-Plasmodium and anti-dengue activities, which appear to be mediated through Csp_P -produced stable bioactive factors with transmission-blocking and therapeutic potential.
References
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