Journal ArticleDOI
Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice
Dusko Ilic,Yasuhide Furuta,Satoshi Kanazawa,Naoki Takeda,Kenji Sobue,Norio Nakatsuji,S Nomura,Jiro Fujimoto,Masato Okada,Tadashi Yamamoto +9 more
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TLDR
Surprisingly, the number of focal adhesions was increased in FAK-deficient cells, suggesting that FAK may be involved in the turnover of focalAdhesion contacts during cell migration.Abstract:
The intracellular protein tyrosine kinase FAK (focal adhesion kinase) was originally identified gy its high level of tyrosine phosphorylation in v-src-transformed cells. FAK is also highly phosphorylated during early development. In cultured cells it is localized to focal adhesion contacts and becomes phosphorylated and activated in response to integrin-mediated binding of cells to the extracellular matrix, suggesting an important role in cell adhesion and/or migration. We have generated FAK-deficient mice by gene targeting to examine the role of FAK during development. Mutant embryos displayed a general defect of mesoderm development, and cells from these embryos had reduced mobility in vitro. Surprisingly, the number of focal adhesions was increased in FAK-deficient cells, suggesting that FAK may be involved in the turnover of focal adhesion contacts during cell migration.read more
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Cellular functions regulated by src family kinases
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References
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TL;DR: The eukaryotic protein kinases make up a large superfamily of homologous proteins, and a classification scheme can be founded on a kinase domain phylogeny, which reveals families of enzymes that have related substrate specificities and modes of regulation.
Journal ArticleDOI
Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase
TL;DR: It is shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes SH2-domain-mediated association of the GRB2 adaptor protein and the c-Src protein-tyrosine kinase (PTK) with FAK in vivo, and also results in activation of mitogen-activated protein Kinase (MAPK).
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Teratocarcinomas and embryonic stem cells : a practical approach
TL;DR: The results allowed us to assess the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds.
Journal ArticleDOI
pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.
TL;DR: The isolation of a cDNA encoding a protein, pp125, that is a major phosphotyrosine-containing protein in untransformed chicken embryo cells and exhibits an increase in phosphotYrosine in pp60v-src-transformedChicken embryo cells is reported.
Journal ArticleDOI
Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly.
TL;DR: A role for integrin- mediated tyrosine phosphorylation in the organization of the cytoskeleton as cells adhere to the extracellular matrix is suggested.