Journal ArticleDOI
Reduction III binding of [14C] aflatoxin B1 to rat liver macromolecules by phenobarbitone pretreatment
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TLDR
The protective effect of PB contrasts with in vitro findings that AFB 1 metabolism is increased by this enzyme-inducing agent, both to detoxification products and to AFB 1 2, 3-oxide, the probable ultimate carcinogenic form of this compound.About:
This article is published in Biochemical Pharmacology.The article was published on 1975-08-15. It has received 64 citations till now. The article focuses on the topics: Aflatoxin & Intraperitoneal injection.read more
Citations
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In vivo covalent binding of organic chemicals to DNA as a quantitative indicator in the process of chemical carcinogenesis.
TL;DR: A comparison of CBI for rat-liver DNA with hepatocarcinogenic potency reveals a surprisingly good quantitative correlation and refineements for a DNA-binding assay are proposed.
Journal ArticleDOI
Evidence for cytochrome P-450NF, the nifedipine oxidase, being the principal enzyme involved in the bioactivation of aflatoxins in human liver.
T Shimada,F P Guengerich +1 more
TL;DR: In vitro studies with human liver indicate that the major catalyst involved in the bioactivation of the hepato-carcinogen aflatoxin B1 to its genotoxic 2,3-epoxide derivative is cytochrome P-450NF, a previously characterized protein that also catalyzes the oxidation of nifedipine and other dihydropyridines, quinidine, macrolide antibiotics, various steroids, and other compounds.
Journal ArticleDOI
Activation and detoxication of aflatoxin B1
TL;DR: Studies with human hepatocytes indicate a major role for GST M1-1 in AFB1 conjugation and that the model chemoprotective agent oltipraz can act by both inducing GSTs and inhibiting P450s.
Journal Article
2,3-Dihydro-2-(guan-7-yl)-3-hydroxy-aflatoxin B1, a Major Acid Hydrolysis Product of Aflatoxin B1-DNA or -Ribosomal RNA Adducts Formed in Hepatic Microsome-mediated Reactions and in Rat Liver in Vivo
TL;DR: DNA- and ribosomal RNA-bound aflatoxin B 1 adducts obtained from salmon sperm DNA and rat liver ribOSomal RNA with fortified rat and hamster liver microsomes were hydrolyzed with weak acid to yield 2,3-dihydro-2-(guan-7-yl)-3-hydroxy-aflatoxin B1 as the major product.
Journal Article
Temporal Patterns of Covalent DNA Adducts in Rat Liver after Single and Multiple Doses of Aflatoxin B1
Robert G. Croy,Gerald N. Wogan +1 more
TL;DR: Administration of multiple doses of aflatoxin B1, using a regimen shown to produce a high incidence of hepatocellular carcinoma, caused accumulation of these persistent products in liver DNA over a 14-day period.
References
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Journal Article
Pharmacological implications of microsomal enzyme induction
TL;DR: It is of considerable interest that certain inducers of liver microsomal enzymes have recently been used therapeutically for the treatment of hyperbilirubinemia in jaundiced children and for thetreatment of Cushing's syndrome.
Journal Article
Liver microsomal metabolism of aflatoxin B 1 to a reactive derivative toxic to Salmonella typhimurium TA 1530.
TL;DR: It is tentatively suggested that the derivative that is toxic to S. typhimurium TA 1530 and the one that reacts with nucleic acids are identical and may be related to the hepatocarcinogenicity of aflatoxin B 1.
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Aflatoxin B1-2,3-oxide: evidence for its formation in rat liver in vivo and by human liver microsomes in vitro.
TL;DR: The data indicate that approximately 60% of the nucleic acid adducts were derived from reactions in vivo with aflatoxin B1-2,3-oxide, which is a probable ultimate carcinogenic metabolite.
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The effect of oral phenobarbitone on hepatic microsomal cytochrome P-450 and demethylation activity in rats fed normal and low protein diets.
W.J. Marshall,A.E.M. Mclean +1 more
TL;DR: A 0.1% solution of sodium phenobarbitone instead of normal drinking water causes maximal stimulation of enzyme activity and an increase in cytochrome P-450, of 7-fold over controls; there are also increases in liver weight and microsomal protein.