Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum.
YongKeun Park,Monica Diez-Silva,Gabriel Popescu,George Lykotrafitis,Wonshik Choi,Michael S. Feld,Subra Suresh +6 more
TLDR
Two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs are investigated and offer potential avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.Abstract:
Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.read more
Citations
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Journal ArticleDOI
Quantitative phase imaging in biomedicine
TL;DR: This Review presents the main principles of operation and representative basic and clinical science applications of quantitative phase imaging, and aims to provide a critical and objective overview of this dynamic research field.
Journal ArticleDOI
Spatial light interference microscopy (SLIM)
Zhuo Wang,Larry J. Millet,Mustafa Mir,Huafeng Ding,Sakulsuk Unarunotai,John A. Rogers,Martha U. Gillette,Gabriel Popescu +7 more
TL;DR: The spatial light interference microscopy (SLIM) as mentioned in this paper is a new optical microscopy technique, capable of measuring nanoscale structures and dynamics in live cells via interferometry.
Journal ArticleDOI
A Multiscale Red Blood Cell Model with Accurate Mechanics, Rheology, and Dynamics
TL;DR: A multiscale RBC model is presented that is able to predict RBC mechanics, rheology, and dynamics in agreement with experiments and based on an analytic theory, the modeled membrane properties can be uniquely related to the experimentally established RBC macroscopic properties without any adjustment of parameters.
Journal ArticleDOI
Optical diffraction tomography for high resolution live cell imaging
Yongjin Sung,Wonshik Choi,Christopher Fang-Yen,Kamran Badizadegan,Ramachandra R. Dasari,Michael S. Feld +5 more
TL;DR: The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.
Spatial light interference microscopy (SLIM)
TL;DR: Spatial light interference microscopy reveals the intrinsic contrast of cell structures and renders quantitative optical path-length maps across the sample, which may prove instrumental in impacting the light microscopy field at a large scale.
References
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PatentDOI
Tomographic phase microscopy
TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.