Journal ArticleDOI
Regeneration of the liverwort Marchantia polymorpha L. From protoplasts isolated from cell suspension culture
TLDR
Callus and regenerated plants (thalli) from protoplasts were obtained by gradually reducing the osmolarity in the medium and subsequently culturing regenerated cells on an inorganic phytohormone-free medium.About:
This article is published in Plant Science Letters.The article was published on 1979-03-01. It has received 69 citations till now. The article focuses on the topics: Marchantia polymorpha & Callus.read more
Citations
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Journal ArticleDOI
Structure and organization of Marchantia polymorpha chloroplast genome. I. Cloning and gene identification.
Kanji Ohyama,Hideya Fukuzawa,Takayuki Kohchi,Tohru Sano,Satoshi Sano,Hiromasa Shirai,Kazuhiko Umesono,Yasuhiko Shiki,Masayuki Takeuchi,Zhen Chang Shin-ichi Aota,Hachiro Inokuchi,Haruo Ozeki +11 more
TL;DR: The complete nucleotide sequence of chloroplast DNA from a liverwort, Marchantia polymorpha, is determined, using a clone bank of chlorOPlast DNA fragments, and the universal genetic code was confirmed by the substitution pattern of simultaneous codons, and by possible codon recognition of thechloroplast-encoded tRNA molecules, assuming no importation of t RNA molecules from the cytoplasm.
Journal ArticleDOI
Convergent evolution of shoots in land plants: lack of auxin polar transport in moss shoots
Tomomichi Fujita,Tomomichi Fujita,Tomomichi Fujita,Hisako Sakaguchi,Hisako Sakaguchi,Yuji Hiwatashi,Yuji Hiwatashi,Steven J. Wagstaff,Motomi Ito,Hironori Deguchi,Toshiyuki Sato,Mitsuyasu Hasebe,Mitsuyasu Hasebe +12 more
TL;DR: In this paper, the soybean auxin-inducible promoter GH3 was fused with a GUS reporter gene and used to indirectly detect auxin distribution in the moss Physcomitrella patens.
Journal ArticleDOI
Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha
Ken Tougane,Kenji Komatsu,Salma Begum Bhyan,Yoichi Sakata,Kimitsune Ishizaki,Katsuyuki T. Yamato,Takayuki Kohchi,Daisuke Takezawa +7 more
TL;DR: It is suggested that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.
Journal ArticleDOI
The Mechanism Forming the Cell Surface of Tip-Growing Rooting Cells Is Conserved among Land Plants
Suvi Honkanen,Victor A.S. Jones,Giulia Morieri,Clément Champion,Alexander J. Hetherington,Steve Kelly,Hélène Proust,Denis Saint-Marcoux,Helen Prescott,Liam Dolan +9 more
TL;DR: It is demonstrated that members of the same orthogroup are active in cell wall synthesis, cell wall integrity sensing, and vesicle trafficking during M. polymorpha rhizoid and Arabidopsis thaliana root hair growth.
Journal ArticleDOI
Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants.
Takefumi Sone,Masaki Fujisawa,Mizuki Takenaka,Saiko Nakagawa,Shohei Yamaoka,Megumi Sakaida,Rie Nishiyama,Katsuyuki T. Yamato,Nobuko Ohmido,Kiichi Fukui,Hideya Fukuzawa,Kanji Ohyama +11 more
TL;DR: There has been structural re-organization of the rDNAs after divergence of the bryophytes from the other plant species in the course of evolution, and this is the first report on co-localized 5S and 45S r DNAs in the rDNA repeat of land plants.
References
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Journal ArticleDOI
Nutritional Requirements for Growth of Vicia hajastana Cells and Protoplasts at a Very Low Population Density in Liquid Media
K. N. Kao,M. R. Michayluk +1 more
TL;DR: Vicia cells were able to grow in a mineral-salt solution supplemented with sucrose (or glucose), a few vitamins, and 2,4-dichlorophenoxyacetic acid but were not able to survive when cultured at a low population density unless the medium was supplemented with zeatin, naphthalene-1-acetic Acid, nucleic-acid bases, amino acids, other sugars, sugar alcohols, and organic acids.
Journal ArticleDOI
Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca
TL;DR: Protoplasts of soybean and N. glauca were induced to fuse with polyethylene glycol (PEG 1540) and up to 39% of the protoplasts in the treated population were heterokaryocytes, which indicated a tendency to stick together and break into pieces in the later cell generations.
Journal ArticleDOI
The production of somatic hybrids by protoplast fusion in the moss, Physcomitrella patens
TL;DR: Somatic hybrids selected following treatment of mixtures of protoplasts from complementary auxotrophic strains with 50 mM CaCl2 at high pH have a morphology different from that of normal haploid strains, but similar to that of aposporously produced diploids.
Journal ArticleDOI
Cell division and plant development from protoplasts of carrot cell suspension cultures.
TL;DR: Cell regeneration and sustained division have been observed in protoplasts from carrot cell suspension cultures, indicating that carrot plants were produced from the protoplast by embryogenesis.
Journal ArticleDOI
Cell division in newly formed cells from protoplasts of soybean.
TL;DR: Washing out the enzyme using conditioned medium plus sorbitol and sucrose as osmotic stabilizers allowed the protoplasts to regenerate new walls and many of the reconstituted cells underwent repeated division.
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