Regulation of growth-defense balance by the JASMONATE ZIM-DOMAIN (JAZ)-MYC transcriptional module.
Ian T. Major,Yuki Yoshida,Marcelo Lattarulo Campos,George Kapali,Xiu Fang Xin,Koichi Sugimoto,Dalton de Oliveira Ferreira,Sheng Yang He,Gregg A. Howe +8 more
Reads0
Chats0
TLDR
It is demonstrated that MYC TFs exert master control over a JAZ‐repressible transcriptional hierarchy that governs growth–defense balance and further showed that triterpenoid biosynthetic and glucosinolate catabolic genes are up‐regulated in jazQ independently of MYCTFs.Abstract:
The plant hormone jasmonate (JA) promotes the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins to relieve repression on diverse transcription factors (TFs) that execute JA responses. However, little is known about how combinatorial complexity among JAZ-TF interactions maintains control over myriad aspects of growth, development, reproduction, and immunity. We used loss-of-function mutations to define epistatic interactions within the core JA signaling pathway and to investigate the contribution of MYC TFs to JA responses in Arabidopsis thaliana. Constitutive JA signaling in a jaz quintuple mutant (jazQ) was largely eliminated by mutations that block JA synthesis or perception. Comparison of jazQ and a jazQ myc2 myc3 myc4 octuple mutant validated known functions of MYC2/3/4 in root growth, chlorophyll degradation, and susceptibility to the pathogen Pseudomonas syringae. We found that MYC TFs also control both the enhanced resistance of jazQ leaves to insect herbivory and restricted leaf growth of jazQ. Epistatic transcriptional profiles mirrored these phenotypes and further showed that triterpenoid biosynthetic and glucosinolate catabolic genes are up-regulated in jazQ independently of MYC TFs. Our study highlights the utility of genetic epistasis to unravel the complexities of JAZ-TF interactions and demonstrates that MYC TFs exert master control over a JAZ-repressible transcriptional hierarchy that governs growth-defense balance.read more
Citations
More filters
Journal ArticleDOI
Modularity in Jasmonate Signaling for Multistress Resilience.
TL;DR: This work reviews the jasmonate signaling pathway with an emphasis on understanding how transcriptional responses are specific, tunable, and evolvable and explores emerging evidence that JAZ proteins integrate multiple informational cues and mediate crosstalk by propagating changes in protein-protein interaction networks.
Journal ArticleDOI
The Crosstalks Between Jasmonic Acid and Other Plant Hormone Signaling Highlight the Involvement of Jasmonic Acid as a Core Component in Plant Response to Biotic and Abiotic Stresses.
TL;DR: It is concluded that jasmonic acid interacts with other hormone signaling pathways to regulate plant growth, abiotic stress tolerance, and defense resistance against hemibiotrophic pathogens such as Magnaporthe oryzae and Pseudomonas syringae.
Journal ArticleDOI
Plant cell wall-mediated immunity: cell wall changes trigger disease resistance responses
TL;DR: The identification of these CWI sensors and PRR-DAMP pairs will help to understand the immune functions of the wall monitoring system, and might allow the breeding of crop varieties and the design of agricultural strategies that would enhance crop disease resistance.
Journal ArticleDOI
Resolution of growth-defense conflict: mechanistic insights from jasmonate signaling.
TL;DR: Evidence is reviewed that diminished growth, as an integral facet of induced resistance, may optimize the temporal and spatial expression of defense compounds without compromising other critical roles of central metabolism, and new insights into the evolution of jasmonate signaling are suggested.
Journal ArticleDOI
Jasmonates are signals in the biosynthesis of secondary metabolites — Pathways, transcription factors and applied aspects — A brief review
Claus Wasternack,Miroslav Strnad +1 more
TL;DR: M modes of action of JAs in the biosynthesis of anthocyanins, nicotine, TIAs, glucosinolates and artemisinin are described and the central role of the SCFCOI1-JAZ co-receptor complex in JA perception and MYB-type and MYC-type transcription factors is described.
References
More filters
Journal ArticleDOI
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources.
TL;DR: By following this protocol, investigators are able to gain an in-depth understanding of the biological themes in lists of genes that are enriched in genome-scale studies.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
Differential expression analysis for sequence count data.
Simon Anders,Wolfgang Huber +1 more
TL;DR: A method based on the negative binomial distribution, with variance and mean linked by local regression, is proposed and an implementation, DESeq, as an R/Bioconductor package is presented.
Journal ArticleDOI
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy
TL;DR: In this paper, the extinction coefficients for chlorophylls a and b in diethylether (Smith, J.H. and Benitez, A.V., eds.), used in this paper as primary standards, were verified by magnesium determination using atomic absorbance spectrophotometry.
Journal ArticleDOI
JAZ repressor proteins are targets of the SCF COI1 complex during jasmonate signalling
Bryan Thines,Leron Katsir,Maeli Melotto,Yajie Niu,Ajin Mandaokar,Guanghui Liu,Kinya Nomura,Sheng Yang He,Gregg A. Howe,John Browse +9 more
TL;DR: The results suggest a model in which jasmonate ligands promote the binding of the SCFCOI1 ubiquitin ligase to and subsequent degradation of the JAZ1 repressor protein, and implicate theSCFCOi1–JAZ1 protein complex as a site of perception of the plant hormone JA–Ile.
Related Papers (5)
The Arabidopsis bHLH Transcription Factors MYC3 and MYC4 Are Targets of JAZ Repressors and Act Additively with MYC2 in the Activation of Jasmonate Responses
Patricia Fernández-Calvo,Andrea Chini,Gemma Fernández-Barbero,José-Manuel Chico,Selena Gimenez-Ibanez,Jan Geerinck,Dominique Eeckhout,Fabian Schweizer,Marta Godoy,José Manuel Franco-Zorrilla,Laurens Pauwels,Erwin Witters,Erwin Witters,María Isabel Puga,Javier Paz-Ares,Alain Goossens,Philippe Reymond,Geert De Jaeger,Roberto Solano +18 more