Journal ArticleDOI
Release of lignin from kraft pulp by a hyperthermophilic xylanase from Thermatoga maritima
Chih-Cheng Chen,Ryan Adolphson,Jeffrey F. D. Dean,Karl-Erik Eriksson,Michael W. W. Adams,Janet Westpheling +5 more
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TLDR
Comparison of the T. maritima recombinant enzyme with a commercially available xylanase, Pulpzyme, indicated that the hyperthermophilic enzyme has several advantages that make it an attractive biotechnological reagent.About:
This article is published in Enzyme and Microbial Technology.The article was published on 1997-01-01. It has received 55 citations till now. The article focuses on the topics: Xylanase & Kraft process.read more
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Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability
Claire Vieille,Gregory J. Zeikus +1 more
TL;DR: This review concentrates on the remarkable thermostability of hyperthermophilic enzymes, and describes the biochemical and molecular properties of these enzymes, which are typically thermostable and optimally active at high temperatures.
Journal ArticleDOI
Developments in industrially important thermostable enzymes: a review.
TL;DR: The source microorganisms and properties of thermostable starch hydrolysing amylases, xylanases, cellulases, chitinases, proteases, lipases and DNA polymerases are discussed and the industrial needs for such specific thermostably enzyme and improvements required to maximize their application in the future are suggested.
Journal ArticleDOI
Molecular and biotechnological aspects of xylanases
TL;DR: Many lines of evidence suggest that xylanases operate via a double displacement mechanism in which the anomeric configuration is retained, although some of the enzymes catalyze single displacement reactions with inversion of configuration.
Journal ArticleDOI
Extremophiles as a source of novel enzymes for industrial application.
TL;DR: This review will briefly discuss the biotechnological significance of extreme thermophilic and hyperthermophilic archaea and bacteria and selected extracellular-polymer-degrading enzymes with potential use in food, chemical and pharmaceutical industries and in environmental biotechnology.
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Potential and utilization of thermophiles and thermostable enzymes in biorefining
TL;DR: existing and potential applications of thermophiles and thermostable enzymes with focus on conversion of carbohydrate containing raw materials are discussed and strategies that enhance thermostablity of enzymes both in vivo and in vitro are assessed.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.