Journal ArticleDOI
Restricted expression of human T-cell leukemia--lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes.
Saira Salahuddin,Phillip D. Markham,Flossie Wong-Staal,Genoveffa Franchini,V. S. Kalyanaraman,Robert C. Gallo +5 more
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TLDR
Multiple immortalized, non-producer, human umbilical cord blood lymphocyte cultures developed by cocultivation or fusion of fresh cells with T cells cultured from leukemia-lymphoma patients are described.About:
This article is published in Virology.The article was published on 1983-08-01. It has received 330 citations till now. The article focuses on the topics: Cell culture & Virus.read more
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Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS
Robert C. Gallo,Syed Zaki Salahuddin,Mikulas Popovic,Gene M. Shearer,Mark H. Kaplan,Barton F. Haynes,Thomas J. Palker,Robert R. Redfield,James M. Oleske,Bijan Safai,Gilbert C. White,Paul Foster,Phillip D. Markham +12 more
TL;DR: Peripheral blood lymphocytes from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS) were grown in vitro with added T-cell growth factor and assayed for the expression and release of human T-lymphotropic retroviruses (HTLV).
Journal ArticleDOI
Trans-Acting Transcriptional Activation of the Long Terminal Repeat of Human T Lymphotropic Viruses in Infected Cells
TL;DR: It is shown that marked differences exist in the ability of LTR sequences of two subtypes of human T lymphotropic virus to function as transcriptional elements in differentiated cell types and that trans-acting regulatory factors present in HTLV-infected cells stimulate gene expression directed by these L TR sequences in a type-specific manner.
Journal ArticleDOI
Inhibition of HIV-1 Replication by a Nonnucleoside Reverse Transcriptase Inhibitor
Vincent J. Merluzzi,K. D. Hargrave,M. Labadia,Grozinger Karl G,Mark T. Skoog,Joe C. Wu,Cheng-Kon Shih,K. Eckner,S. Hattox,Julian Adams +9 more
TL;DR: Dipyridodiazepinones inhibition of HIV-1 replication in vitro as demonstrated by in situ hybridization, inhibition of protein p24 production, and the lack of syncytia formation in cultured human T cell lines and freshly isolated human peripheral blood lymphocytes.
Journal ArticleDOI
Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS
Jörg Schüpbach,Mikulas Popovic,Raymond V. Gilden,Matthew A. Gonda,M. G. Sarngadharan,Robert C. Gallo +5 more
TL;DR: Another subgroup of HTLV, designated HTLV-III, has now been isolated from many patients with AIDS and pre-AIDS and is shown to be a true member of the HTLV family.
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The trans-activator gene of the human T cell lymphotropic virus type III is required for replication
TL;DR: It is concluded that the tat-III gene product is required for efficient replication of HTLV-III in T4+ cells, and for that reason is important for the cytopathic effects of virus infection.
References
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
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Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma
Bernard J. Poiesz,Francis W. Ruscetti,Adi F. Gazdar,Paul A. Bunn,John D. Minna,Robert C. Gallo +5 more
TL;DR: The number of these particle-associated proteins is consistent with the expected proteins of a retrovirus, but the sizes of some are distinct from those of most known retroviruses of the primate subgroups.
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Cytochemical Identification of Monocytes and Granulocytes
L. T. Yam,C. Y. Li,W. H. Crosby +2 more
TL;DR: Cytochemical methods for chloroacetate esterase, nonspecific esterases, peroxidase, and metachromasia were used to identify monocytes and neutrophilic, basophilic, or eosinophilic granulocytes.
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Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.
TL;DR: It is indirectly shown that all or at least a major population of human thymus-derived lymphocytes under certain conditions will form nonimmune rosettes with sheep red blood cells (SRBC), and it is suggested that these ro settes are formed by a rapidly released or metabolized receptor substance on the living cell surface which behaves as a trypsin-sensitive structure produced by the cells themselves.