Journal ArticleDOI
Review of NEDDylation inhibition activity detection methods
Shu-Yu Wang,Xu Liu,Yuan Liu,Hong-Yu Zhang,Yan-Bing Zhang,Chong Liu,Jian Song,Jin-Bo Niu,Sai-Yang Zhang +8 more
TLDR
This review will provide an up-to-date and comprehensive review of the methods for detecting NEDDylation activity that will contribute to NEDdylation inhibitor development.About:
This article is published in Bioorganic & Medicinal Chemistry.The article was published on 2021-01-01. It has received 3 citations till now. The article focuses on the topics: Neddylation & Protein ubiquitination.read more
Citations
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Journal ArticleDOI
Discovery of 1,2,4-triazine dithiocarbamate derivatives as NEDDylation agonists to inhibit gastric cancers
Jian Song,Yuan Liu,Xin-Ying Yuan,Wen-Bo Liu,Yin-Ru Li,Guang-Xi Yu,Xin-Yi Tian,Yan-Bing Zhang,Xiang-Jing Fu,Sai-Yang Zhang,Sai-Yang Zhang +10 more
TL;DR: NEDDylation promotion induced by compound K3 is reported, which could inhibit the cancer cell lines MGC-803 and HGC-27 and induce thecancer cell apoptosis via prompting the degradation of c-IAP and YAP/TAZ.
Journal ArticleDOI
Discovery of novel indole derivatives that inhibit NEDDylation and MAPK pathways against gastric cancer MGC803 cells.
Dong-Jun Fu,Xin-Xin Cui,Ting Zhu,Yan-Bing Zhang,Yang-Yang Hu,Li-Rong Zhang,Sheng-Hui Wang,Sai-Yang Zhang +7 more
TL;DR: A series of novel indole derivatives were synthesized and evaluated for their antiproliferative activity against three selected cancer cell lines (MGC803, EC-109 and PC-3) as discussed by the authors.
Journal ArticleDOI
Targeting protein modifications in metabolic diseases: molecular mechanisms and targeted therapies
TL;DR: A comprehensive review of protein posttranslational modification (PTM) and its role in metabolic diseases and pathological consequences is presented in this paper , where the authors afford a through description of proteins and pathways involved in metabolic disease by focusing on PTM-based protein modifications.
References
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Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
TL;DR: Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.
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Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
TL;DR: Bacterial adsorbent not only had a distinct advantage in speed of antigen isolation, but analyses by polyacrylamide gel electrophoresis in SDS also revealed consistently higher antigen recoveries, lower levels of background radioactivity, and an absence of other cell components which may nonspecifically bind to and complicate analyses using conventional immune precipitates.
Journal ArticleDOI
RING Domain E3 Ubiquitin Ligases
TL;DR: RING E3s have been linked to the control of many cellular processes and to multiple human diseases, and knowledge of the physiological partners, biological functions, substrates, and mechanism of action for most RING E 3s remains at a rudimentary stage.
Journal ArticleDOI
An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer
Teresa A. Soucy,Peter G. Smith,Michael Milhollen,Allison Berger,James M. Gavin,Sharmila Adhikari,James E. Brownell,Kristin E. Burke,Cardin David P,Stephen Critchley,Courtney Cullis,Amanda Doucette,James J. Garnsey,Gaulin Jeffrey L,Rachel E. Gershman,Anna R. Lublinsky,Alice McDonald,Hirotake Mizutani,Usha Narayanan,Edward J. Olhava,Stephane Peluso,Mansoureh Rezaei,Michael D. Sintchak,Tina Talreja,Michael P. Thomas,Tary Traore,Stepan Vyskocil,Jie Yu,Julie Zhang,Lawrence R. Dick,Christopher F. Claiborne,Mark Rolfe,Joseph B. Bolen,Steven P. Langston +33 more
TL;DR: MLN4924 disrupts cullin-RING ligase-mediated protein turnover leading to apoptotic death in human tumour cells by a new mechanism of action, the deregulation of S-phase DNA synthesis, suggesting that NAE inhibitors may hold promise for the treatment of cancer.