Journal ArticleDOI
Salivary Glucose Oxidase: Multifunctional Roles for Helicoverpa zea?
TLDR
The labial glands of Helicoverpa zea produced a glucose oxidase (GOX), which was present in the saliva and midgut lumen and appears to have similar kinetic and physical attributes to other insect GOXs.Abstract:
Labial glands of Helicoverpa zea produced a glucose oxidase (GOX), which was present in the saliva and midgut lumen. We purified GOX 23-fold by isoelectric focusing of labial gland homogenates and investigated physical and kinetic properties of the enzyme. D-glucose and 6-deoxy-D-glucose were the optimal substrates of 22 carbohydrates tested with GOX. The enzyme was not inhibited by several inhibitors of fungal GOX but was sensitive to HgCl(2). Labial gland GOX activities varied daily during larval development with highest activities found when larvae were actively feeding. Based on pH optimum, pI, molecular weight estimate and K(m(glucose)), the insect enzyme is not derived from fungal GOXs but appears to have similar kinetic and physical attributes to other insect GOXs. Some possible functions are discussed, including antimicrobial properties, manipulating midgut O(2) levels, altering host plant defense responses, and metabolizing carbohydrates. Arch. Copyright 1999 Wiley-Liss, Inc.read more
Citations
More filters
Journal ArticleDOI
The Myriad Plant Responses to Herbivores.
TL;DR: Differential expression of plant genes in response to closely related insect species suggest that some elicitors generated by phloem-feeding insects are species-specific and are dependent on the herbivore's developmental stage.
Journal ArticleDOI
Specialist versus generalist insect herbivores and plant defense
Jared G. Ali,Anurag Agrawal +1 more
TL;DR: Although a novel set of predictions based on current coevolutionary hypotheses are outlined, few consistencies in plant induction by specialists versus generalists, feeding guilds are predictive of differential plant responses.
Journal ArticleDOI
Plant Defense against Insect Herbivores
TL;DR: Both plant defense and insect adaptation involve metabolic costs, so most plant-insect interactions reach a stand-off, where both host and herbivore survive although their development is suboptimal.
Journal ArticleDOI
Plant resistance towards insect herbivores : a dynamic interaction
TL;DR: Both plant defence and insect adaptation involve a metabolic cost, and in a natural system most plant-insect interactions involving herbivory reach a 'stand-off' where both host and herbivore survive but develop suboptimally.
Journal ArticleDOI
Herbivory: caterpillar saliva beats plant defences.
Richard O. Musser,Sue M. Hum-Musser,Herbert Eichenseer,Michelle Peiffer,Gary N. Ervin,J. Brad Murphy,Gary W. Felton +6 more
TL;DR: It is found that the enzyme glucose oxidase counteracts the production of nicotine induced by the caterpillar feeding on the plant, and is responsible for suppressing the defensive reaction they induce in their hosts.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
An Elicitor of Plant Volatiles from Beet Armyworm Oral Secretion
Hans T. Alborn,Ted C. J. Turlings,Ted C. J. Turlings,Ted C. J. Turlings,T. H. Jones,T. H. Jones,T. H. Jones,G. Stenhagen,G. Stenhagen,G. Stenhagen,John H. Loughrin,John H. Loughrin,John H. Loughrin,James H. Tumlinson,James H. Tumlinson,James H. Tumlinson +15 more
TL;DR: The compound N -(17-hydroxylinolenoyl)-l-glutamine (named here volicitin) was isolated from oral secretions of beet armyworm caterpillars and induced the seedlings to emit volatile compounds that attract parasitic wasps and natural enemies of the herbivores.
Journal ArticleDOI
beta-Glucosidase: an elicitor of herbivore-induced plant odor that attracts host-searching parasitic wasps
TL;DR: Findings show that beta-glucosidase is a P. brassicae-secreted elicitor of the defense response of cabbage plants to herbivore injury, inducing the emission of volatiles that are used by parasitoids of the herbivores to locate their victims.