Journal ArticleDOI
Screening ofExiguobacterium acetylicum from soil samples showing enantioselective and alkalotolerant esterase activity
TLDR
Two enantioselective strains were selected by the ratio of initial rate of hydrolysis of enantiomerically pure (R)- and (S)-2-phenylbutyric acid resorufin ester (2PB-O-res) as substrates and showed high esterase activity, but showed low lipase activity onp-nitrophenyl palmitate (pNPP).Abstract:
About 3,000 bacterial colonies with esterase activities were isolated from soil samples by enrichment culture and halo-size on Luria broth-tributyrin (LT) plates. The colonies were assayed for esterase activity in microtiter plates using enantiomerically pure (R)- and (S)-2-phenylbutyric acid resorufin ester (2PB-O-res) as substrates. Two enantioselective strains (JH2 and JH13) were selected by the ratio of initial rate of hydrolysis of enantiomerically pure (R)- and (S)-2-PB-O-res. When cell pellets were used, both strains showed hgh apparent enantioselectivity (E
app>100) for (R)-2PB-O-res and were identified asExiguobacterium acetylicum. The JH13 strain showed high esterase activity onp-nitrophenyl acetate (pNPA), but showed low lipase activity onp-nitrophenyl palmitate (pNPP). The esterase was located in the soluble fraction of the cell extract. The crude intracellular enzyme preparation was stable at a pH range from 6.0 to 11.0.read more
Citations
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Journal ArticleDOI
The Exiguobacterium genus: biodiversity and biogeography.
TL;DR: Different molecular approaches for the comparative analysis of 24 isolates from markedly diverse environments including ancient Siberian permafrost and hot springs at Yellowstone National Park suggested that Exiguobacterium strains formed two distinct divisions that generally agreed with temperature ranges for growth.
Journal ArticleDOI
Novel plant growth promoting rhizobacteria—Prospects and potential
TL;DR: Plant growth promoting rhizobacteria are an important group of microbial inoculants that have been studied extensively for their ability to promote plant growth and improve productivity and the information available on these novel PGPRs with regard to their biology and utility is discussed in this review.
Journal ArticleDOI
Effect of alkaline protease-producing Exiguobacterium sp. YS1 inoculation on the solubilization and bacterial community of waste activated sludge
TL;DR: Bioaugmentation of the organism might be useful for enhancing the solubilization of WAS at mild alkaline pH, indicating a synergistic effect could be achieved when both bacterial inoculation and alkaline treatment were combined.
Journal ArticleDOI
Functional annotation of hypothetical proteins from the Exiguobacterium antarcticum strain B7 reveals proteins involved in adaptation to extreme environments, including high arsenic resistance.
Wana Lailan Oliveira da Costa,Carlos Leonardo Araújo,Larissa M. Dias,Lino César Pereira,Jorianne Thyeska Castro Alves,Fabricio Araujo,Edson Luiz Folador,Isabel Henriques,Artur Silva,Adriana Ribeiro Carneiro Folador +9 more
TL;DR: This study aimed to assign functions to proteins previously described as HPs, present in the genome of E. antarcticum B7, to produce a better understanding of the mechanism by which this bacterium adapts to extreme environments and to the finding of targets with biotechnological interest.
Journal ArticleDOI
Comparative Genomics Analysis of a New Exiguobacterium Strain from Salar de Huasco Reveals a Repertoire of Stress-Related Genes and Arsenic Resistance.
Juan Castro-Severyn,Francisco Remonsellez,Sandro L. Valenzuela,Cesar Salinas,Jonathan Fortt,Pablo Aguilar,Coral Pardo-Esté,Cristina Dorador,Raquel Quatrini,Franck Molina,Daniel Aguayo,Eduardo Castro-Nallar,Claudia P. Saavedra +12 more
TL;DR: Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant, and expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp.
References
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Journal ArticleDOI
Quantitative analyses of biochemical kinetic resolutions of enantiomers
Book ChapterDOI
Enzymatic reactions in organic media
TL;DR: This work states that large scale and industrial nonaqueous enzymatic conversions of synthetic interest and modes of using enzymes in organic media, including Oxidoreductases and other non-hydrolases, are of great interest.
Journal ArticleDOI
Enzymes in the synthesis of chiral drugs.
TL;DR: The use of enzymes in the synthesis and modification of optically pure drugs is reviewed, with special attention on the synthesis of new pharmaceuticals which may require efficient procedures for large-scale synthesis in the future.
Journal ArticleDOI
Ferulic acid release and 4-vinylguaiacol formation during brewing and fermentation: indications for feruloyl esterase activity in Saccharomyces cerevisiae.
TL;DR: The release of ferulic acid and the subsequent thermal or enzymatic decarboxylation to 4-vinylguaiacol are inherent to the beer production process and suggests the activity of feruloyl esterases produced by brewer's yeast is suggested.
Journal ArticleDOI
Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures.
Evangelos Topakas,Haralambos Stamatis,Peter Biely,Dimitris Kekos,B.J. Macris,Paul Christakopoulos +5 more
TL;DR: Determination of k(cat)/K(m) revealed that the enzyme hydrolysed methyl sinapinate 6, 21 and 40 times more efficiently than methyl ferulate, methyl coumarate and methyl caffeate, respectively, indicating a synergistic interaction between FAE-II and S. thermophile xylanase.