Separation of progressive motile sperm from mouse semen using on-chip chemotaxis.
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TLDR
This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection.Abstract:
We present a novel method for the separation of progressive motile sperm from non-progressive motile and immotile sperm. This separation was accomplished by inducing chemotaxis along a longitudinal chemical gradient in a microchip composed of a biocompatible polydimethysiloxane layer and a glass substrate. In a preliminary experiment using fluorescent rhodamine B as a marker, we verified that a chemical gradient was generated by diffusion within the microchannel. We used acetylcholine as a chemoattractant to evaluate the chemotactic response of sperm. We tested the response to a 1/2 to 1/64 dilution series of acetylcholine. The results of a mouse sperm chemotaxis assay showed that progressive motile sperm swam predominantly toward the outlet at an optimal chemical gradient of 0.625 (mg/ml)/mm of acetylcholine. This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection.read more
Citations
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Chemotactic behavior of spermatozoa captured using a microfluidic chip.
TL;DR: By facilitating measurement of sperm response in ascending, descending, and uniform chemoattractant concentration, the assay could isolate sperm chemotactic response from rheotaxis and chemokinesis and was validated using acetylcholine and further tested with rat oviductal fluid from the estrus phase.
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Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip.
Jingchun Li,Bolin Ning,Cao Xinyan,Yinghua Luo,Guo Li,Wei Guosheng,Liu Shengjun,Ying Zhang,Zhang Aizhong,Wu Rui,Yanbing Li +10 more
TL;DR: The results showed that separated sperm using 7.5-mm microchannel chip were improved in sperm motility, swimming velocity, and beat frequency compared with other groups, and the optimized microchip system provides a good opportunity to sort motile bull sperm for IVF.
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Microfluidic Devices Developed for and Inspired by Thermotaxis and Chemotaxis.
Alireza Karbalaei,Hyoung J. Cho +1 more
TL;DR: Various microfluidic devices that have either been used for, or inspired by thermo- and chemotaxis are reviewed categorically.
References
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A novel 3D mammalian cell perfusion-culture system in microfluidic channels
Yi-Chin Toh,Chi Zhang,Jing Zhang,Yuet Mei Khong,Shi Chang,Victor Samper,Danny van Noort,Dietmar W. Hutmacher,Hanry Yu +8 more
TL;DR: A transparent 3D microfluidic channel-based cell culture system that allows cells to be perfusion-cultured in 3D by supporting them with adequate 3D cell-cell and cell-matrix interactions and allows direct optical monitoring of cellular events for a wide range of applications.