Serial Quantitative PCR Assay for Detection, Species Discrimination, and Quantification of Leishmania spp. in Human Samples
Jason L. Weirather,Selma M. B. Jeronimo,Shalini Gautam,Shyam Sundar,Mitchell Kang,Melissa A. Kurtz,Rashidul Haque,Albert Schriefer,Sinésio Talhari,Edgar M. Carvalho,John E. Donelson,Mary E. Wilson +11 more
Reads0
Chats0
TLDR
Serial qPCR is useful for leishmania detection and species determination and for absolute quantification when compared to a standard curve from the same Leishmania species.Abstract:
The invention provides a method for determining the presence, species, and/or quantity of Leishmania in a sample.read more
Citations
More filters
Journal ArticleDOI
Report of a WHO Expert Committee. (WHO Technical Report Series No. 807), VI. World Health Organization, Geneva (1991), 65 pages, Soft cover SFr. 11
Journal ArticleDOI
Leishmania infections: Molecular targets and diagnosis
Mohammad Akhoundi,Tim Downing,Jan Votýpka,Katrin Kuhls,Julius Lukeš,Arnaud Cannet,Christophe Ravel,Pierre Marty,Pascal Delaunay,Mohamed Kasbari,Bruno Granouillac,Luigi Gradoni,Denis Sereno +12 more
TL;DR: A classification method based on the suitability of well-studied molecular markers for typing the 21 known Leishmania species pathogenic to humans is proposed, which can be applied to newly discovered species and to hybrid strains originating from inter-species crosses.
Journal ArticleDOI
Cutaneous and mucocutaneous leishmaniasis : Differential diagnosis, diagnosis, histopathology, and management
TL;DR: The diagnosis of leishmaniasis can be challenging because it mimics both infectious and malignant conditions as discussed by the authors, and a misdiagnosis may lead to an unfavorable outcome, and appropriate management requires an accurate diagnosis, which often includes identification of the specific etiologic species.
Journal ArticleDOI
Species typing in dermal leishmaniasis.
TL;DR: This review gives an overview of the currently existing and most used assays for species discrimination, with a critical appraisal of the limitations of each technique.
Journal ArticleDOI
Real-time PCR applications for diagnosis of leishmaniasis.
TL;DR: This review summarizes the qPCR-based methods in the diagnosis of leishmaniasis focusing on the recent developments and applications in this field.
References
More filters
Book ChapterDOI
Primer3 on the WWW for general users and for biologist programmers.
Steve Rozen,Helen Skaletsky +1 more
TL;DR: This chapter assumes acquaintance with the principles and practice of PCR, as outlined in, for example, refs.
Journal ArticleDOI
Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.
Diane Pennica,Glenn Evan Nedwin,Joel S. Hayflick,Peter H. Seeburg,Rik Derynck,Michael A. Palladino,William J. Kohr,Bharat B. Aggarwal,David V. Goeddel +8 more
TL;DR: Recombinant tumour necrosis factor can be obtained by expression of its complementary DNA in Escherichia coli and induces the haemorrhagic necrosis of transplanted methylcholanthrene-induced sarcomas in syngeneic mice.
Journal ArticleDOI
Visceral leishmaniasis: what are the needs for diagnosis, treatment and control?
François Chappuis,François Chappuis,Shyam Sundar,Asrat Hailu,H. W. Ghalib,Suman Rijal,Rosanna W. Peeling,Jorge Alvar,Marleen Boelaert +8 more
TL;DR: Millefosine, paromomycin and liposomal amphotericin B are gradually replacing pentavalent antimonials and conventional amphoteric in B as the preferred treatments in some regions, but in other areas these drugs are still being evaluated in both mono- and combination therapies.
Journal ArticleDOI
Drug Resistance in Leishmaniasis
TL;DR: It is essential that there be a strategy to prevent the emergence of resistance to new drugs; combination therapy, monitoring of therapy, and improved diagnostics could play an essential role in this strategy.
Journal ArticleDOI
Leishmania and human immunodeficiency virus coinfection: the first 10 years.
Jorge Alvar,Carmen Cañavate,Beatriz Gutiérrez-Solar,Maribel Jiménez,Fernando Laguna,Rogelio López-Vélez,Ricardo Molina,Javier Moreno +7 more
TL;DR: Over 850 Leishmania-human immunodeficiency virus (HIV) coinfection cases have been recorded, the majority in Europe, where 7 to 17% of HIV-positive individuals with fever have amastigotes, suggesting that Leishmanniasis-infected individuals without symptoms will express symptoms of leishmaniasis if they become immunosuppressed.