Luca Galluzzi

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: A real-time PCR-based assay for rapid detection of all toxic species of the Alexandrium genus in both fixative-preserved environmental samples and cultures is developed, confirming that real- time PCR could be a valid, fast alternative procedure for the detection and quantification of target phytoplankton species during coastal water monitoring.

TL;DR: The potential for determining embryo sex directly from blastocoele fluid is demonstrated by amplifying the multicopy genes TSPY1 and TBC1D3, opening up the possibility of screening embryos from couples carrying an X-linked disorder to identify male embryos at high risk of disease.

TL;DR: Similarities in composition of the phytoplankton community were evidenced, with a clear dominance of dinoflagellates over the other taxa in the Mediterranean basin, as well as in two harbours studied.

TL;DR: The results showed a general variability in the rRNA gene content depending on the strain and, for the species A. taylori, in relation also to the growth phase, which should be taken into account for the application of the real-time quantitative PCR-based techniques for monitoring purposes in coastal seawaters.