Signaling regulates activity of DHCR24, the final enzyme in cholesterol synthesis.
Winnie Luu,Eser J. Zerenturk,Ika Kristiana,Martin P. Bucknall,Laura J. Sharpe,Andrew J. Brown +5 more
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TLDR
The data indicate a novel mechanism whereby DHCR24 activity is regulated by signaling, and determined the effect of known phosphorylation sites and found that mutating certain residues inhibited DH CR24 activity.About:
This article is published in Journal of Lipid Research.The article was published on 2014-03-01 and is currently open access. It has received 50 citations till now. The article focuses on the topics: Phosphorylation & Kinase.read more
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Flux analysis of cholesterol biosynthesis in vivo reveals multiple tissue and cell-type specific pathways
TL;DR: Stable isotope labeling and isotopomer analysis is used to trace sterol flux through the Bloch and Kandutsch-Russell pathways in mice to permit maintenance of two interdigitated pathways permits production of diverse bioactive sterols that can be regulated independently of cholesterol.
Journal ArticleDOI
DHCR7: A vital enzyme switch between cholesterol and vitamin D production.
TL;DR: This review summarizes current knowledge about the critical enzyme DHCR7, highlighting recent findings regarding its structure, transcriptional and post-transcriptional regulation, and its links to vitamin D synthesis.
Journal ArticleDOI
Cholesterol-mediated Degradation of 7-Dehydrocholesterol Reductase Switches the Balance from Cholesterol to Vitamin D Synthesis *
TL;DR: It is identified that statin treatment can ameliorate the low DHCR7 expression seen with common Smith-Lemli-Opitz syndrome mutations, and it is shown that wild-typeDHCR7 is also relatively labile, highlighting DH CR7 as an important regulatory switch between cholesterol and vitamin D synthesis.
Journal ArticleDOI
The UPS and downs of cholesterol homeostasis.
TL;DR: Recent work uncovering the ubiquitylation and degradation of key players in cholesterol homeostasis is reviewed, including the low-density lipoprotein (LDL) receptor, transcription factors, flux-controlling enzymes in cholesterol synthesis, and cholesterol exporters.
Journal ArticleDOI
The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally.
TL;DR: DHCR7 is important for both cholesterol and vitamin D synthesis, and a novel layer of regulation is identified, whereby its activity is controlled by DHCR24, which suggests the existence of a cholesterol “metabolon”, where enzymes from the same metabolic pathway interact with each other to provide a substrate channeling benefit.
References
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Journal ArticleDOI
The Pfam protein families database
Marco Punta,Penny Coggill,Ruth Y. Eberhardt,Jaina Mistry,John Tate,Chris Boursnell,Ningze Pang,Kristoffer Forslund,Goran Ceric,Jody Clements,Andreas Heger,Liisa Holm,Erik L. L. Sonnhammer,Sean R. Eddy,Alex Bateman,Robert D. Finn +15 more
TL;DR: The definition and use of family-specific, manually curated gathering thresholds are explained and some of the features of domains of unknown function (also known as DUFs) are discussed, which constitute a rapidly growing class of families within Pfam.
Journal ArticleDOI
The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C.
D Toullec,P Pianetti,H Coste,P Bellevergue,T Grand-Perret,M Ajakane,V Baudet,P Boissin,E Boursier,F Loriolle +9 more
TL;DR: GF 109203X was a competitive inhibitor with respect to ATP and displayed high selectivity for PKC as compared to five different protein kinases, illustrating the potential of this compound as a tool for studying the involvement of PKC in signal transduction pathways.
Journal ArticleDOI
Scansite 2.0: proteome-wide prediction of cell signaling interactions using short sequence motifs
TL;DR: Scansite identifies short protein sequence motifs that are recognized by modular signaling domains, phosphorylated by protein Ser/Thr- or Tyr-kinases or mediate specific interactions with protein or phospholipid ligands, allowing segments of biological pathways to be constructed in silico.
Book ChapterDOI
Receptor-mediated endocytosis of low-density lipoprotein in cultured cells
TL;DR: Study of the cell surface binding, internalization, and metabolism of low-density lipoprotein (LDL) in cultured cells have provided useful information regarding the general aspects of receptor-mediated endocytosis and three classes of mutant alleles at the LDL receptor locus have been deduced.
Journal ArticleDOI
Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells.
Takashi Chijiwa,Atsushi Mishima,Masatoshi Hagiwara,Mamoru Sano,Kyozo Hayashi,Tsutomu Inoue,Naito Kenji,Tadashi Toshioka,Hiroyoshi Hidaka +8 more
TL;DR: In this article, a newly synthesized isoquinolinesulfonamide, H-89 (N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline-sulfoneamide), was shown to have a potent and selective inhibitory action against cyclic AMP-dependent protein kinase (protein kinase A), with an inhibition constant of 0.048 +/- 0.008 microM.