Single-exposure optical focusing inside scattering media using binarized time-reversed adapted perturbation.
Cheng Ma,Fengbo Zhou,Yan Liu,Lihong V. Wang +3 more
- Vol. 2, Iss: 10, pp 869-876
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TLDR
This work rapidly measuring the perturbed optical field within a single camera exposure followed by adaptively time-reversing the phase-binarized perturbation produces a phase-conjugated wavefront synthesized within a millisecond, two orders of magnitude shorter than the digitally achieved record.Abstract:
Light scattering inhibits high-resolution optical imaging, manipulation, and therapy deep inside biological tissue by preventing focusing. To form deep foci, wavefront-shaping techniques that break the optical diffusion limit have been developed. For in vivo applications, such focusing must provide a high gain, high speed, and a high focal peak-to-background ratio. However, none of the previous techniques meet these requirements simultaneously. Here, we overcome this challenge by rapidly measuring the perturbed optical field within a single camera exposure followed by adaptively time-reversing the phase-binarized perturbation. Consequently, a phase-conjugated wavefront is synthesized within a millisecond, two orders of magnitude shorter than the digitally achieved record. We demonstrate real-time focusing in dynamic scattering media and extend laser speckle contrast imaging to new depths. The unprecedented combination of a fast response, high gain, and high focusing contrast makes this work a major stride toward in vivo deep-tissue optical imaging, manipulation, and therapy.read more
Citations
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Journal ArticleDOI
Focusing light inside dynamic scattering media with millisecond digital optical phase conjugation.
TL;DR: This work develops a simpler but faster DOPC system that focuses light not only through, but also inside scattering media, and is an important step toward in vivo deep-tissue non-invasive optical imaging, manipulation, and therapy.
Journal ArticleDOI
Deep tissue optical focusing and optogenetic modulation with time-reversed ultrasonically encoded light.
Haowen Ruan,Joshua Brake,J. Elliott Robinson,Yan Liu,Mooseok Jang,Cheng Xiao,Chunyi Zhou,Viviana Gradinaru,Changhuei Yang +8 more
TL;DR: The first example of TRUE focusing in 2-mm-thick living brain tissue is presented and it is found that TRUE focusing enabled precise control of neuron firing and increased the spatial resolution of neuronal excitation fourfold when compared to conventional lens focusing.
Journal ArticleDOI
In vivo study of optical speckle decorrelation time across depths in the mouse brain.
Muhammad Mohsin Qureshi,Joshua Brake,Hee-Jae Jeon,Haowen Ruan,Yan Liu,Abdul Mohaimen Safi,Tae Joong Eom,Changhuei Yang,Euiheon Chung +8 more
TL;DR: By examining the scattering dynamics in the mouse brain in vivo via multispeckle diffusing wave spectroscopy (MSDWS) using a custom fiber probe that simulates a point-like source within the brain, the relationship between this decorrelation time and the depth of the point- like light source inside the living mouse brain is investigated.
Journal ArticleDOI
Focusing light through biological tissue and tissue-mimicking phantoms up to 9.6 cm in thickness with digital optical phase conjugation.
TL;DR: The demonstrated penetration of nearly 10 cm (∼100 transport mean free paths) has never been achieved before by any optical focusing technique, and it shows the promise of OPC for deep-tissue noninvasive optical imaging, manipulation, and therapy.
PatentDOI
Optical focusing inside scattering media with time-reversed ultrasound microbubble encoded (trume) light
TL;DR: This paper presents a new technique, time-reversed ultrasound microbubble encoded (TRUME) optical focusing, which can focus light with improved efficiency and sub-ultrasound wavelength resolution, and demonstrates a twofold enhancement in addressable focus resolution in a microbuble aggregate target by exploiting the nonlinear pressure-to-destruction response of the microbubbles.
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