Solution NMR: A powerful tool for structural and functional studies of membrane proteins in reconstituted environments
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TLDR
Current approaches and technological developments in the determination of membrane protein structures by solution NMR are discussed and recent structural and mechanistic insights gained with this technique are highlighted.About:
This article is published in Journal of Biological Chemistry.The article was published on 2019-11-01 and is currently open access. It has received 50 citations till now. The article focuses on the topics: Membrane protein & Nanodisc.read more
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Journal ArticleDOI
The structural basis of T-cell receptor (TCR) activation: An enduring enigma
TL;DR: The molecular basis for TCR activation is examined in light of the recently determined cryoEM structure of a complete TCR–CD3 complex, and major knowledge gaps are identified that must be filled to arrive at a comprehensive model of TCRactivation that explains how pMHC-specific information is transmitted across the T-cell membrane to initiate intracellular signaling.
Journal ArticleDOI
Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches.
TL;DR: This review assesses some of the more general effects that a membrane environment exerts on lipid bilayer-embedded proteins such as GPCRs, followed by the consideration of more specific effects, including stoichiometric interactions with specific lipid subtypes.
Book ChapterDOI
Neuropathy target esterase (NTE/PNPLA6) and organophosphorus compound-induced delayed neurotoxicity (OPIDN)
Rudy J. Richardson,John K. Fink,John K. Fink,Paul Glynn,Robert B. Hufnagel,Galina F. Makhaeva,Sanjeeva J. Wijeyesakere +6 more
TL;DR: Systemic inhibition of neuropathy target esterase with certain organophosphorus compounds produces OP compound-induced delayed neurotoxicity (OPIDN), a distal degeneration of axons in the central nervous system and peripheral nervous system, thereby providing a powerful model for studying a spectrum of neurodegenerative diseases.
Journal ArticleDOI
Bicelles and nanodiscs for biophysical chemistry.
TL;DR: Membrane nanoobjects are very important tools to study biomembrane properties as they can be used to stabilize membrane protein assemblies to be studied by electron microscopy and advantages and drawbacks of both nanoplatforms are discussed.
Journal ArticleDOI
Lipid Membrane Mimetics in Functional and Structural Studies of Integral Membrane Proteins
TL;DR: The most widely used membrane mimetics in structural and functional studies of integral membrane proteins (IMPs) are detergents, liposomes, bicelles, nanodiscs/Lipodisqs, amphipols, and lipidic cubic phases as discussed by the authors.
References
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Book
NMR of proteins and nucleic acids
TL;DR: The NMR Assignment Problem in Biopolymers, two-Dimensional NMR With Proteins and Nucleic Acids, and Sequence-Specific Resonance Assignments.
Book
Principles of nuclear magnetic resonance in one and two dimensions
TL;DR: In this paper, the dynamics of nuclear spin systems were studied by two-dimensional exchange spectroscopy and nuclear magnetic resonance imaging (NEMI) imaging, and two different correlation methods based on coherence transfer were proposed.
Journal ArticleDOI
Diffusion of Univalent Ions Across the Lamellae of Swollen Phospholipids
TL;DR: It is found that as the surface charge of the lipid lamellae is increased, the amount of cation per μmle of lipid increases, and the phospholipid liquid crystalline structures appear to “bind” or “capture” cations.
Journal ArticleDOI
Drug Discovery: A Historical Perspective
TL;DR: The biotech industry is establishing itself as the discovery arm of the pharmaceutical industry, and in bridging the gap between academia and large pharmaceutical companies, the biotech firms have been effective instruments of technology transfer.