Journal ArticleDOI
Specific interaction between the self-splicing RNA of Tetrahymena and its guanosine substrate: implications for biological catalysis by RNA
Brenda L. Bass,Thomas R. Cech +1 more
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Splicing of the ribosomal RNA precursor of Tetrahymena has previously been shown to require no protein in vitro; the cleavage–ligation activity is intrinsic to the RNA molecule, and analysis of the reaction kinetics suggests that guanosine binds to a specific site on the pre-rRNA.Abstract:
Splicing of the ribosomal RNA precursor of Tetrahymena has previously been shown to require no protein in vitro; the cleavage-ligation activity is intrinsic to the RNA molecule. Analysis of the reaction kinetics with guanosine, which is a substrate in the reaction, and with several guanosine analogues suggests that guanosine binds to a specific site on the pre-rRNA. It appears that the RNA, like an enzyme, binds its substrate to promote the rate and specificity of a biological reaction.read more
Citations
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The noncoding RNA revolution-trashing old rules to forge new ones.
TL;DR: The pathway of ncRNA research is described, where every established "rule" seems destined to be overturned.
Journal ArticleDOI
Why nature chose phosphates
TL;DR: Stable, negatively charged phosphates react under catalysis by enzymes; organic chemists, who can only rarely use enzymatic catalysis for their reactions, need more highly reactive intermediates than phosphates.
Patent
Rna ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods.
TL;DR: In this paper, a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA) is used to synthesize a set of sequence-specific endoribonucleases.
Journal ArticleDOI
The structural and functional diversity of metabolite-binding riboswitches.
Adam Roth,Ronald R. Breaker +1 more
TL;DR: A diverse set of metabolite-sensing RNAs is found to exploit a variety of distinct mechanisms to regulate genes that are fundamental to metabolism.
Journal ArticleDOI
The intervening sequence RNA of Tetrahymena is an enzyme.
Arthur J. Zaug,Thomas R. Cech +1 more
TL;DR: The shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena thermophila acts as an enzyme in vitro that can act as an RNA polymerase, differing from the protein enzyme in that it uses an internal rather than an external template.
References
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Journal ArticleDOI
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme
TL;DR: The RNA moieties of ribonuclease P purified from both E. coli and B. subtilis can cleave tRNA precursor molecules in buffers containing either 60 mM Mg2+ or 10 mM MG2+ plus 1 mM spermidine, and in vitro, the RNA and protein subunits from one species can complement sub units from the other species in reconstitution experiments.
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Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena
Kelly Kruger,Paula J. Grabowski,Arthur J. Zaug,Julie Sands,Daniel E. Gottschling,Thomas R. Cech +5 more
TL;DR: It is proposed that the IVS portion of the RNA has several enzyme-like properties that enable it to break and reform phosphodiester bonds and that enzymes, small nuclear RNAs and folding of the pre-rRNA into an RNP are unnecessary for these reactions.
Journal ArticleDOI
In vitro splicing of the ribosomal RNA precursor of tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequence
TL;DR: It is proposed that Tetrahymena pre-rRNA splicing occurs by a phosphoester transferase mechanism where the guanosine cofactor provides the free 3' hydroxyl necessary to initiate a series of three transfers that results in splicing of the pre- rRNA and cyclization of the excised IVS.
Journal ArticleDOI
Secondary structure of 16S ribosomal RNA
Harry F. Noller,Carl R. Woese +1 more
TL;DR: A secondary structure model for 16S ribosomal RNA which is based on available chemical, enzymatic, and comparative sequence data shows good agreement between constraints dictated by the model and a wide variety of experimental observations.