State of Adenovirus 2 Deoxyribonucleic Acid in the Nucleus and Its Mode of Transcription: Studies with Isolated Viral Deoxyribonucleic Acid-Protein Complexes and Isolated Nuclei
Robert D. Wallace,Joseph Kates +1 more
TLDR
Newly replicated adenovirus 2 deoxyribonucleic acid (DNA) can be isolated from the nucleus of HeLa cells by a gentle lysis procedure as a fairly homogeneous complex with a sedimentation of 73S and the synthesis of virus-specific RNA by the isolated nuclei is strongly inhibited by low doses of alpha-amanitine.Abstract:
Newly replicated adenovirus 2 deoxyribonucleic acid (DNA) can be isolated from the nucleus of HeLa cells by a gentle lysis procedure as a fairly homogeneous complex with a sedimentation of 73S. The viral DNA complex can be prepared completely free from host cell DNA. The viral complex is slightly active in ribonucleic acid (RNA) synthesis in vitro. Treatment of the complex with Pronase and sodium dodecyl sulfate converts the DNA to a form which sediments at 43S. Nuclei isolated from adeno-infected cells synthesize high-molecular-weight virus-specific RNA in vitro. Optimal RNA synthesis requires a divalent cation, preferentially manganese, and relatively high salt concentrations. The synthesis of virus-specific RNA by the isolated nuclei is strongly inhibited by low doses of alpha-amanitine. The latter experimental result is discussed in terms of the polymerase used to transcribe the adenovirus DNA in vivo.read more
Citations
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An amazing sequence arrangement at the 5′ ends of adenovirus 2 messenger RNA
TL;DR: Findings imply a new mechanism for the biosynthesis of Ad2 mRNA in mammalian cells which is complementary to sequences within the Ad2 genome which are remote from the DNA from which the main coding sequence of each mRNA is transcribed.
Journal ArticleDOI
Selective and accurate initiation of transcription at the ad2 major late promotor in a soluble system dependent on purified rna polymerase ii and dna
TL;DR: Specific transcription initiation at the late promoter is observed with extracts derived from either virus-infected or uninfected KB cells and with class II RNA polymerases isolated from either human calf, murine or amphibian cells.
Journal ArticleDOI
Promoters and heterogeneous 5' termini of the messenger RNAs of adenovirus serotype 2.
Carl C. Baker,Edward B. Ziff +1 more
TL;DR: A model for Ad2 cap site recognition is proposed in which RNA polymerase scans short DNA segments for purine starts, and is discussed in the context of EIV mRNAs of Ad2 and Ad5, which differ in their promoter region DNA sequence.
Journal ArticleDOI
Transcripts from the adenovirus-2 major late promoter yield a single early family of 3′ coterminal mRNAs and five late families
Alan R. Shaw,Edward B. Ziff +1 more
TL;DR: It is shown that a major event in the Ad-2 early-late switch is a novel form of control which activates L2-L5 mRNA production, and implies that precisely the same transcription start site is utilized for early L1 mRNA synthesis as is used during the late stage for L1-L 5 late mRNA synthesis.
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A new species of virus-coded low molecular weight RNA from cells infected with adenovirus type 2
TL;DR: A virus-coded low molecular weight RNA (5.2S), which migrates slightly faster on polyacrylamide gels than the well characterized adenovirus-specific 5.5S RNA, has been isolated from cells infected with adenOVirus type 2.
References
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