Journal ArticleDOI
Specific Inhibition of Nuclear RNA Polymerase II by α-Amanitin
TLDR
α-Amanitin, a toxic substance from the mushroom Amanita phalloides, is a potent inhibitor of DNA-dependent RNA polymerase II (the nucleoplasmic form) from sea urchin, rat liver, and calf thymus.Abstract:
alpha-Amanitin, a toxic substance from the mushroom Amanita phalloides, is a potent inhibitor of DNA-dependent RNA polymerase II (the nucleoplasmic form) from sea urchin, rat liver, and calf thymus. This compound exerts no effect on the activity of polymerase I (nucleolar form) or polymerase III (also nucleoplasmic). The inhibition is due to a specific interaction with polymerase II or with a complex of DNA and polymerase II.read more
Citations
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Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription.
TL;DR: It is shown that recruitment of the p160 class of coactivators is sufficient for gene activation and for the growth stimulatory actions of estrogen in breast cancer supporting a model in which ER cofactors play unique roles in estrogen signaling.
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A critical period for macromolecular synthesis in long-term heterosynaptic facilitation in Aplysia
P.G. Montarolo,Philip Goelet,Vincent F. Castellucci,J A Morgan,Eric R. Kandel,Samuel Schacher +5 more
TL;DR: The results parallel those for behavioral performance in vertebrates and indicate that the critical time window characteristic of the requirement for macromolecular synthesis in long-term heterosynaptic facilitation is not a property of complex circuitry, but an intrinsic characteristic of specific nerve cells and synaptic connections involved in the long- term storage of information.
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A block to elongation is largely responsible for decreased transcription of c-myc in differentiated HL60 cells.
David Bentley,Mark Groudine +1 more
TL;DR: During HL60 differentiation, a DNase I hypersensitive site in the chromatin about 300 bases downstream of the 5′ end of of intron 1 increases in intensity relative to other sites, possibly reflecting events associated with the termination of transcription.
Journal ArticleDOI
Chromatin structure analyses identify miRNA promoters
Fatih Ozsolak,Laura L. Poling,Zhengxin Wang,Hui Liu,X. Shirley Liu,Robert G. Roeder,Xinmin Zhang,Jun S. Song,David E. Fisher +8 more
TL;DR: The high-throughput identification of miRNA promoter and enhancer regulatory elements sheds light on evolution of mi RNA transcription and permits rapid identification of transcriptional networks of miRNAs.
Journal ArticleDOI
The dynamics of a pre-mRNA splicing factor in living cells.
TL;DR: It is concluded that one function of speckles is to supply splicing factors to active genes, demonstrating that the interphase nucleus is far more dynamic in nature than previously assumed.
References
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Journal ArticleDOI
Multiple Forms of DNA-dependent RNA Polymerase in Eukaryotic Organisms
TL;DR: Three distinct RNA polymerase activities have been isolated from developing sea urchin embryos, and in rat liver nuclei there are two RNA polymerases activities.
Journal ArticleDOI
α-Amanitin: A specific inhibitor of one of two DNA-dependent RNA polymerase activities from calf thymus
Claude Kedinger,Claude Kedinger,M. Gniazdowski,M. Gniazdowski,Jean-Louis Mandel,Jean-Louis Mandel,F. Gissinger,F. Gissinger,Pierre Chambon,Pierre Chambon +9 more
TL;DR: α-amanitin selectively inhibits RNA synthesis catalyzed by calf thymus RNA polymerase activity B by interacting with the enzyme and inhibiting chain elongation as discussed by the authors, which can be used to identify the source of RNA synthesis.
Journal ArticleDOI
Specific Nucleolar and Nucleoplasmic RNA Polymerases
TL;DR: It is concluded that the RNA polymerases are specifically localized within the nucleus and may, therefore, play specific roles in the regulation of genetic transcription.
Journal ArticleDOI
Poisonous Principles of Mushrooms of the Genus Amanita Four-carbon amines acting on the central nervous system and cell-destroying cyclic peptides are produced
Journal ArticleDOI
Studies on the stimulation by ammonium sulphate of the DNA-dependent RNA polymerase of isolated rat-liver nuclei.
C.C. Widnell,Jamshed R. Tata +1 more
TL;DR: It is inferred that nuclei incubated in the absence of ammonium sulphate may synthesize ribosomal RNA, and that the action of ammonia sulphate is to induce the synthesis of an RNA more DNA-like in its base composition.