Journal ArticleDOI
Stem Cell Properties of Human Dental Pulp Stem Cells
Stan Gronthos,Jaime S. Brahim,W. Li,Larry W. Fisher,Natasha Cherman,Alan Boyde,Pamela DenBesten,P. Gehron Robey,Songtao Shi +8 more
TLDR
It is demonstrated that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation, including single-colony-derived DPSC strains that differ from each other with respect to their rate of odontogenesis.Abstract:
In this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and re-transplanted into immunocompromised mice to generate a dentin-pulp-like tissue, demonstrating their self-renewal capability. DPSCs were also found to be capable of differentiating into adipocytes and neural-like cells. The odontogenic potential of 12 individual single-colony-derived DPSC strains was determined. Two-thirds of the single-colony-derived DPSC strains generated abundant ectopic dentin in vivo, while only a limited amount of dentin was detected in the remaining one-third. These results indicate that single-colony-derived DPSC strains differ from each other with respect to their rate of odontogenesis. Taken together, these results demonstrate that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation.read more
Citations
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Journal ArticleDOI
Investigation of multipotent postnatal stem cells from human periodontal ligament
Byoung Moo Seo,Masako Miura,Stan Gronthos,P. M. Bartold,Sara Batouli,Jaime S. Brahim,M. F. Young,Pamela Gehron Robey,Cun-Yu Wang,Songtao Shi +9 more
TL;DR: It is suggested that PDL contains stem cells that have the potential to generate cementum/PDL-like tissue in vivo and transplantation of these cells might hold promise as a therapeutic approach for reconstruction of tissues destroyed by periodontal diseases.
Journal ArticleDOI
SHED: Stem cells from human exfoliated deciduous teeth
Masako Miura,Stan Gronthos,Mingrui Zhao,Bai Lu,Larry W. Fisher,Pamela Gehron Robey,Songtao Shi +6 more
TL;DR: It is shown that a naturally exfoliated human organ contains a population of stem cells that are completely different from previously identified stem cells, which may be an unexpected unique resource for stem-cell therapies including autologous stem- cell transplantation and tissue engineering.
Journal ArticleDOI
Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources: Their Biology and Role in Regenerative Medicine
TL;DR: This article will review the isolation and characterization of the properties of different dental MSC-like populations in comparison with those of other MSCs, such as BMMSCs.
Journal ArticleDOI
Mesenchymal Stem Cells: Revisiting History, Concepts, and Assays
TL;DR: The experimental origin of the concept of the population(s) referred to as mesenchymal stem cells and the experimental framework required to assess their stemness and function are revisited.
Journal ArticleDOI
Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp.
Songtao Shi,Stan Gronthos +1 more
TL;DR: The finding that BMSSCs and DPSCs both display phenotypes consistent with different perivascular cell populations, regardless of their diverse ontogeny and developmental potentials, may have further implications in understanding the factors that regulate the formation of mineralized matrices and other associated connective tissues.
References
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Journal ArticleDOI
Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues
TL;DR: Marrow stromal cells present an intriguing model for examining the differentiation of stem cells and have several characteristics that make them potentially useful for cell and gene therapy.
Journal ArticleDOI
Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
TL;DR: Postnatal human DPSCs are isolated that have the ability to form a dentin/pulp-like complex and are compared with human bone marrow stromal cells, known precursors of osteoblasts.
Journal ArticleDOI
Bone Marrow Stromal Stem Cells: Nature, Biology, and Potential Applications
TL;DR: Bone marrow stromal cells are progenitors of skeletal tissue components such as bone, cartilage, the hematopoiesis‐supporting stroma, and adipocytes and represent an important paradigm of post‐natal nonhematopoietic stem cells, and an easy source for potential therapeutic use.
Journal ArticleDOI
Isolation of multipotent adult stem cells from the dermis of mammalian skin
Jean G. Toma,Mahnaz Akhavan,Karl J. L. Fernandes,Fanie Barnabé-Heider,Abbas F. Sadikot,David R. Kaplan,Freda D. Miller +6 more
TL;DR: The isolation of stem cells from juvenile and adult rodent skin is described and it is proposed that these cells represent a novel multipotent adult stem cell and suggest that skin may provide an accessible, autologous source of stem cell for transplantation.
Journal ArticleDOI
The Evolving Concept of a Stem Cell: Entity or Function?
TL;DR: This work was supported by a Lutheran Fellowship and an NIH predoctoral training grant GM07149 to T.B.R. and by NIH grants CA59717, AG09521, HD 18179, and HL65572 to H.M. Reid.