Journal ArticleDOI
Stimulation of germination of dormant oat (Avena sativa L.) seeds by ethanol and other alcohols
TLDR
The results obtained seem to demonstrate that the stimulatory action of ethanol on germination of dormant oat caryopses requires its metabolism through ADH and involves an activation of glycolysis and the Krebs cycle.Abstract:
Dormant dehusked Avena sativa L. (cv. Moyencourt) caryopses germinated easily at 10°C, but their germination was poor at 25 and 30°C. Ethanol overcame their dormancy and allowed germination at the last two temperatures when given continuously at concentrations ranging from 50 to 200 mm, or at higher concentrations at the beginning of imbibition. Ethanol also promoted germination in hypoxia. Other alcohols which were good substrates for alcohol dehydrogenase (ADH: EC 1.1.1.1), such as butanol-1, propanol-1 and 2-propen-1-ol, had the same stimulatory effect as ethanol, whereas alcohols which could not be oxidized by ADH (propanol-2 and methanol) did not improve germination. Salicylhydroxamic acid (SHAM) did not alter the stimulation of germination induced by ethanol, but 4-methylpyrazole (4-MP, an inhibitor of ADH) completely abolished this stimulation. The improvement of germination by alcohols which were good substrates for ADH was always associated with an increase in oxygen uptake by caryopses, whereas alcohols which were not ADH substrates did not enhance respiration. The stimulation of oxygen uptake induced by ethanol resulted in a decrease in CO2/O2 ratio and was suppressed by 4-MP, but ATP level in embryos was not modified until after the germination of caryopses. All the results obtained seem to demonstrate that the stimulatory action of ethanol on germination of dormant oat caryopses requires its metabolism through ADH and involves an activation of glycolysis and the Krebs cycle.read more
Citations
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Journal ArticleDOI
ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation.
Krystyna Oracz,Hayat El-Maarouf Bouteau,Jill M. Farrant,Keren Cooper,Maya Belghazi,Claudette Job,Dominique Job,F. Corbineau,Christophe Bailly +8 more
TL;DR: In this paper, the role of reactive oxygen species (ROS) in after-ripening of sunflower seeds was investigated at the cellular level by electron microscopy, concomitantly with lipid peroxidation and oxidation of specific embryo proteins.
Journal ArticleDOI
Role of protein and mRNA oxidation in seed dormancy and germination.
TL;DR: In this paper, the role of reactive oxygen species (ROS) in the regulation of seed germination and dormancy has been investigated and the cellular basis of their action remains unknown, but very challenging to elucidate due to the lack of specificity of these compounds that can potentially react with all biomolecules.
Journal ArticleDOI
The regulation of secondary dormancy. The membrane hypothesis revisite
TL;DR: Dormancy cycling is paralleled by modulations of germination responsiveness to germination stimulants, such as light and nitrate and to the width of the germination temperature window, which has implications for the perception of temperature.
Journal ArticleDOI
Problemática e manejo de bancos de sementes de invasoras em solos agrícolas
TL;DR: The problematic of weed seed banks in the soil is discussed in terms of its importance, the mechanisms that guarantee the maintenance of large seed numbers in the surface of the soil and the dynamics of Weed seed banks.
Journal ArticleDOI
Physiological mechanisms for potato dormancy release and sprouting: a review.
TL;DR: Potato ( Solanum tuberosum L.) tubers undergo a period of dormancy, during which visible bud growth is inhibited and two phytohormones, ABA and ethylene suppress tuber sprouting; however, the exact role of ethylene remains to be elucidated.
References
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A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate.
TL;DR: A procedure allowing one to measure fructose 2,6-bisphosphate in amounts lower than a picomole, is described and the kinetic properties of the purified enzyme have been investigated.
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