Stoichiometric conversion of oxygen to superoxide anion during the respiratory burst in neutrophils. Direct evidence by a new method for measurement of superoxide anion with diacetyldeuteroheme-substituted horseradish peroxidase.
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It is suggested that O-2, formed during phagocytosis, is converted to H2O2 within phagosomes and then diffuses out into the extracellular medium when its decomposition by catalase and/or peroxidases is blocked by azide.About:
This article is published in Journal of Biological Chemistry.The article was published on 1986-09-05 and is currently open access. It has received 129 citations till now. The article focuses on the topics: Superoxide & Respiratory burst.read more
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Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing.
TL;DR: Neutrophils are one of the professional phagocytes in humans that ingest bacteria into intracellular spaces and are involved in phagocytosis.
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Hydrogen peroxide: a signaling messenger.
James R. Stone,Suping Yang +1 more
TL;DR: This review evaluates the evidence that H2O2 functions as a signaling agent in higher organisms in light of the known biology and biochemistry of H2 O2 and identifies oxidation-dependent steps in signal transduction pathways being uncovered.
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Oxidation of methionyl residues in proteins: tools, targets, and reversal.
TL;DR: Considering the reversibility of Met oxidation and the functional changes associated with the oxidation, it seems possible that Met oxidation/reduction in proteins may be one means to control homeostasis in biological systems.
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The neutrophil NADPH oxidase
TL;DR: The recent identification of homologs of one of the membrane components in nonphagocytic cells will expand understanding of the biological contexts in which this system may function.
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Neutrophil activation on biological surfaces. Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes.
TL;DR: The results suggest that PMN adherent to intra- or extravascular surfaces may undergo a massive, prolonged respiratory burst at the command of macrophages and lymphocytes reacting to microbial products and antigens.
References
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Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN)
Joe M. McCord,Irwin Fridovich +1 more
TL;DR: The demonstration that O2·- can reduce ferricytochrome c and tetranitromethane, and that superoxide dismutase, by competing for the superoxide radicals, can markedly inhibit these reactions, is demonstrated.
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Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.
TL;DR: O(2) (-) is made by leukocytes under circumstances which suggest that it may be involved in bacterial killing, and is identified as the agent responsible for the leukocyte-mediated reduction of cytochrome c.
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Oxygen-dependent microbial killing by phagocytes (second of two parts).
TL;DR: Since Metschnikoff's discovery, hundreds of scientists studying dozens of species have reported thousands of studies on these cells, perhaps the most widely recognized of which are those of the eminent English scientists.
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Active Oxygen Species and the Functions of Phagocytic Leukocytes
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H2O2 Release from Human Granulocytes during Phagocytosis: RELATIONSHIP TO SUPEROXIDE ANION FORMATION AND CELLULAR CATABOLISM OF H2O2: STUDIES WITH NORMAL AND CYTOCHALASIN B-TREATED CELLS
Richard K. Root,Julia A. Metcalf +1 more
TL;DR: Results indicate that with normal cells approximately 50% of H(2)O( 2) released during phagocytosis is derived directly from O(2.) (-) by dismutation, the remainder appearing from an (intra)cellular source shared by azide-inhibitable heme enzymes.